Project description:To investigate gene expression changes in fish by the secondary effluent (directly released to environment) of a waterwater treatment plant in Tucson, Arizona, zebrafish larvae with 5-day exposure to the original (1x) or half (0.5x) concentration of the effluent were analyzed using Agilent G2519F-026437 Zebrafish Oligo Microarray.
Project description:This experiment set includes 64 arrays representing 26 serovars and strains of Salmonella spp. including many representatives of subspecies I, Arizona from subsp. IIIa, and S. bongori from subsp. V. The genomic DNA from all strains were labeled with Cy5 and hybridized against an equal amount (1.5 ug) of S. typhimurium SL1344 reference genomic DNA that was labeled with Cy3, all on an S. typhimurium SL1344 spotted DNA microarray. Most of the arrays are present in triplicate to account for variability in probe generation, hybridization, and slide quality. Several are represented in duplicate, and a few without any replicates.
