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MiCLIP in METTL14 R298P mutation knock-in cell clones

ABSTRACT: miCLIP in METTL14 R298P mutation knock-in cell clones

PROVIDER: PRJNA783876 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR17044638.fastq.gz	Fastqsanger.gz
	SRR17044639.fastq.gz	Fastqsanger.gz
	SRR17044640.fastq.gz	Fastqsanger.gz
	SRR17044640_1.fastq.gz	Fastqsanger.gz
	SRR17044640_2.fastq.gz	Fastqsanger.gz

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miCLIP in METTL14 R298P mutation knock-in cell clones

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Project description:N6-methyladenosine (m6A) is the major type of RNA modiﬁcation that regulates RNA stability and gene expression, characterized by a conserved motif including 5′-(m6A)C-3′. However, the functional significance of the specific motif for m6A modification is unclear. Here, we focused on a single amino acid substitution in the m6A “writer” complex, METTL14 R298P, and created mutation knock-in cells. m6A individual-nucleotide-resolution cross-linking and immunoprecipitation and methylated RNA immunoprecipitation with next-generation sequencing (MeRIP-Seq) revealed that METTL14R298P/R298P but not METTL14WT/R298P cells had an aberrant sequence motif, 5′-(m6A)[C/T]-3′. This study reveals a mechanism underlying sequence-specific m6A modification.

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