Ontology highlight
ABSTRACT:
PROVIDER: PRJNA785352 | ENA |
REPOSITORIES: ENA
Similar Datasets
Project description:Plethodontid salamanders are the largest family of salamanders and are classic models for studying the effect of rapidly evolving courtship pheromones on mating behavior and reproductive success. Despite interests in plethodontid reproduction, very little is known about the molecular composition of salamander gametes, as the extraordinary sizes of their genomes have impaired the development of various omic-scale resources. To identify what proteins may be expressed in salamander sperm, we performed DIA-MS on sperm samples from two plethodontid species, Plethodon shermani and Desmognathus ocoee. As the first detailed study of salamander sperm, this study partially fills in a critical taxonomic gap in the study of fertilization proteins in vertebrates.
2022-04-18 | PXD030143 | Pride
Project description:Skin microbiome of CA plethodontid salamanders
| PRJNA434592 | ENA
Project description:We conducted transcriptome sequencing of Clonorchis sinesis sub-organs (sucker, muscle, ovary and testis) with Illumina sequencing.
2013-01-30 | E-MTAB-827 | biostudies-arrayexpress
Project description:In this study, three small RNA libraries constructed from gonad tissues of XX female, XY male and YY super-male yellow catfish were sequenced by Solexa high-throughput sequencing technology to investigate the expression pattern of sex-biased microRNA. The sequencing data generated a total of 384 conserved miRNAs and 113 potential novel miRNAs, among which 23, 30 and 14 miRNAs were specifically detected in XX ovary, XY testis, and YY testis, respectively. Interestingly, more abundant piRNAs were found in ovary compared to testis in yellow catfish, which phenomenon is also observed in other fish species but opposite in mammalians. We detected a number of microRNAs differentially expressed between ovary and testis, such as miR-21, miR-462, miR-430 and -200 family. When compared the transcriptome between XY and YY testis, we observed relative lower expression of miR-141 and miR-429 in YY testis. Histological analysis indicated that YY super-males have more spermatids and less spermatocytes in spermatogenic cyst than XY males under the same age and culturing conditions. The expression level of miR-141 and 429 coincides with the progression of spermatogenesis both in yellow catfish and human. At last, The expression pattern of nine arbitrarily selected miRNAs detected by quantitative RT-PCR was consistent with the Solexa sequencing results. Our study provides a comprehensive miRNA transcriptome analysis for gonad of yellow catfish with different sex genotypes, and identifies a number of sex-biased miRNAs that are potentially involved in sex differentiation and spermatogenesis.
2016-01-24 | GSE54610 | GEO
Project description:M. rosenbergii is a classical aquatic organism with sex-reversed phenomenon by gene-expressed regulation. Reversal of gonad development in M. rosenbergii received great attention but the role of transcription factors for gonadal maintenance and sex determination in adult shrimps remain unclearly. In this study, we sequencing the transcriptomes for the adult testis, ovary and androgenic gland by third-generation sequencing plus second-generation sequencing, and 21,345 were identified. In total, 9,199 differentially expressed genes (DEGs) were identified in pairwise comparison where 2,990 DEGs were upregulated expression in testis than other tissues, and 5,775 DEGs were upregulated expression in ovary, and 2,244 DEGs were upregulated expression in androgenic. There are 272 differentially expressed transcription factors (DETs) in total. The upregulated DETs in one of the testis, ovary and androgenic glands compared to the others were 107, 152 and 13 respectively. The GO and KEGG of DETs analysis suggested that MAPK signaling pathway and transcriptional misregulation pathway have key function in testis, the thyroid hormone signaling pathway and neuroactive ligand-receptor interaction play a important role in ovary. We determined the existence of networks containing significant DETs related to sex determination in the adult goands of M. rosenbergii. The key DETs are Piwi (only expression in testis and ovary) and Argonaute 2 (only expression in ovary) might be involved with the regulation of ovary development.
2021-04-11 | GSE171823 | GEO
Project description:Tissue regeneration is widely distributed across the tree of life. Among vertebrates, salamanders possess an exceptional ability to regenerate amputated limbs and other complex structures. Thus far, molecular insights about limb regeneration have come from a relatively limited number of species from two closely related salamander families. To gain broader perspective on the molecular basis of limb regeneration and enhance the molecular toolkit of an emerging plethodontid salamander (Bolitoglossa ramosi), we used RNA-seq to generate transcript sequence data and identify 602 genes that are differentially expressed during limb regeneration. This list was further processed to identify a core set of genes that exhibit conserved expression changes between B. ramosi and the Mexican axolotl (Ambystoma mexicanum), and presumably their common ancestor approximately 180 million years ago. Our study highlights the importance of developing comparative gene expression data for studies of limb regeneration among salamanders. All animals used in this work were collected under the Contract on Genetic Access for scientific research for non commercial profit (Contrato de acceso a recursos genéticos para la investigación científica sin interés commercial) to Resources number 118–2015.
2018-09-26 | GSE105232 | GEO
Project description:Patiria miniata testis and ovary transcriptome sequencing
| PRJNA175319 | ENA
Project description:Despite increasing efforts in miRNAs identification across various species and diverse tissue types, little is known about porcine gonad-specific miRNAs. Although the well-known importance of pig in agriculture, as well as a model for human biology, the miRNA catalog of pig has been largely undefined. Identification and preliminary characterization of gonad-specific miRNAs would be a prerequisite for a thorough understanding of their roles in regulating folliculogenesis and spermatogenesis. In the present study, we get insight into miRNA transcriptome in adult porcine ovary and testis using deep sequencing technology, and to elucidate their characteristic organ- and gender-specific profiles, genomic context and emphasize the features of X-linked miRNAs. Two small RNA libraries from adult porcine ovary and testis tissues were sequenced.
2011-03-29 | E-GEOD-24443 | biostudies-arrayexpress