DIA-MS of plethodontid salamander sperm

ABSTRACT: Plethodontid salamanders are the largest family of salamanders and are classic models for studying the effect of rapidly evolving courtship pheromones on mating behavior and reproductive success. Despite interests in plethodontid reproduction, very little is known about the molecular composition of salamander gametes, as the extraordinary sizes of their genomes have impaired the development of various omic-scale resources. To identify what proteins may be expressed in salamander sperm, we performed DIA-MS on sperm samples from two plethodontid species, Plethodon shermani and Desmognathus ocoee. As the first detailed study of salamander sperm, this study partially fills in a critical taxonomic gap in the study of fertilization proteins in vertebrates.

INSTRUMENT(S):

ORGANISM(S): Desmognathus Ocoee Plethodon Shermani

TISSUE(S): Spermatozoon

SUBMITTER: Damien Wilburn  

LAB HEAD: Brian Searle

PROVIDER: PXD030143 | Pride | 2022-04-18

REPOSITORIES: Pride