Dataset Information

DIA-MS of plethodontid salamander sperm

ABSTRACT: Plethodontid salamanders are the largest family of salamanders and are classic models for studying the effect of rapidly evolving courtship pheromones on mating behavior and reproductive success. Despite interests in plethodontid reproduction, very little is known about the molecular composition of salamander gametes, as the extraordinary sizes of their genomes have impaired the development of various omic-scale resources. To identify what proteins may be expressed in salamander sperm, we performed DIA-MS on sperm samples from two plethodontid species, Plethodon shermani and Desmognathus ocoee. As the first detailed study of salamander sperm, this study partially fills in a critical taxonomic gap in the study of fertilization proteins in vertebrates.

INSTRUMENT(S): Orbitrap Exploris 480

ORGANISM(S): Desmognathus Ocoee Plethodon Shermani

TISSUE(S): Spermatozoon

SUBMITTER: Damien Wilburn

LAB HEAD: Brian Searle

PROVIDER: PXD030143 | Pride | 2022-04-18

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	2021mar21_mander_600pool_gpfdia_01.raw	Raw
	2021mar21_mander_600pool_gpfdia_02.raw	Raw
	2021mar21_mander_600pool_gpfdia_03.raw	Raw
	2021mar21_mander_600pool_gpfdia_04.raw	Raw
	2021mar21_mander_600pool_gpfdia_05.raw	Raw

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Publications

Recurrent Co-Option and Recombination of Cytokine and Three Finger Proteins in Multiple Reproductive Tissues Throughout Salamander Evolution.

Wilburn Damien B DB Kunkel Christy L CL Feldhoff Richard C RC Feldhoff Pamela W PW Searle Brian C BC

Frontiers in cell and developmental biology 20220223

Reproductive proteins evolve at unparalleled rates, resulting in tremendous diversity of both molecular composition and biochemical function between gametes of different taxonomic clades. To date, the proteomic composition of amphibian gametes is largely a molecular mystery, particularly for Urodeles (salamanders and newts) for which few genomic-scale resources exist. In this study, we provide the first detailed molecular characterization of gametes from two salamander species (<i>Plethodon sher ...[more]

PMID: 35281090

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