A male-derived non-ribosomal peptide pheromone controls female schistosome development II
Ontology highlight
ABSTRACT:
PROVIDER: PRJNA789552 | ENA |
REPOSITORIES: ENA
Dataset's files
| Action | DRS | |||
|---|---|---|---|---|
| SRR17248189.fastq.gz | Fastqsanger.gz | |||
| SRR17248190.fastq.gz | Fastqsanger.gz | |||
| SRR17248191.fastq.gz | Fastqsanger.gz | |||
| SRR17248192.fastq.gz | Fastqsanger.gz | |||
| SRR17248193.fastq.gz | Fastqsanger.gz |
Items per page: 5 1 - 5 of 6 |
Similar Datasets
Project description:A male-derived non-ribosomal peptide pheromone controls female schistosome development
| PRJNA766647 | ENA
Project description:gli1 is a gene in the male S. mansoni required for female sexual development. As a putative transcription factor, RNAseq comparing pairs with gli1 knocked-down or control males was performed to look for the genes which are trancriptionally regulated by gli1. sm-nrps was one of the essential candidate discovered in this analysis that has been characterized in the same publication, revealing a pheromone directed novel mechanism of female schistosome sexual development.
2021-10-04 | GSE184849 | GEO
Project description:βAlanyl-tryptamine (BATT) is a peptide secreted by male S. mansoni upon pairing with a female parasite. Treatment of synthetic BATT on virgin females wihtout any pairing is sufficient to induce sexual development and egg depostiion.
2021-12-23 | GSE191062 | GEO
Project description:The MHC class I antigen presentation system enables T cell immunosurveillance of cancers and viruses. Rapidly degraded nascent polypeptides (DRiPs) provide many class I peptide ligands. By knocking down each of the 80 ribosomal proteins, we identified proteins that modulate peptide generation without altering source protein expression. We show that 60S ribosomal proteins L6 (RPL6) and RPL28, which are adjacent on the ribosome, play opposite roles in generating an influenza A virus encoded peptide. RPL6 depletion decreases ubiquitin-dependent peptide presentation, while RPL28 depletion increases ubiquitin-dependent and -independent peptide presentation. 40S ribosomal protein S28 (RPS28) knockdown increases total peptide supply in uninfected cells by increasing DRiP synthesis from non-canonical translation of “untranslated” regions and non-AUG start codons, and sensitizes tumor cells for T cell targeting. RPL23 knocKDut decreases overall peptide supply, impairing T cell recognition of tumor cells. Our findings raise the possibility of modulating immunosurveillance by pharmacologically targeting ribosomes.
2019-01-21 | GSE114484 | GEO
Project description:Whole genome expression profile comparing MGAS315 treated with XIP pheromone versus vehicle-treated cells Interpretations are described further in the manuscript to be submitted: authors Mashburn-Warren, Morrison, and Federle. Title: The Cryptic Competence Pathway in Streptococcus pyogenes is Controlled by a Peptide Pheromone.
2012-06-01 | GSE37974 | GEO
Project description:To identify female sex pheromone biosynthesis genes by differential expression between males and females. Female pheromone gland tissue and male abdominal tip tissue was compared by RNA-Seq of cDNA. Transcripts were assembled using Trinity and differential expression analysis done with RSEM.
2023-12-31 | E-MTAB-12752 | biostudies-arrayexpress
Project description:Pheromones exchanged by conspecifics are a major class of chemical signals that can alter behavior, physiology, and development. In particular, males and females communicate with potential mating partners via sex pheromones to promote reproductive success. Physiological and developmental mechanisms by which pheromones facilitate progeny production remain largely enigmatic. Here we describe how a C. elegans male pheromone, ascr#10, improves the oogenic germline. Before most signs of aging become evident, C. elegans hermaphrodites start producing lower quality gametes characterized by abnormal morphology, increased rates of chromosomal nondisjunction, and higher penetrance of deleterious alleles. We show that exposure to the male pheromone substantially ameliorates all of these defects and reduces embryonic lethality. ascr#10 stimulates proliferation of germline precursor cells in adult hermaphrodites. Greater precursor supply increases physiological germline cell death, which is required to improve oocyte quality in older mothers. The hermaphrodite germline is sensitive to the pheromone only during a time window, comparable in duration to a larval stage, used by the pre-reproductive adults to assess suitability of the environment for reproduction. Our results identify developmental events that occur in the oogenic germline in response to a male pheromone. They also suggest that the opposite effects of the pheromone on gamete quality and organismal longevity arise from a competition over resource allocation between soma and the germline.
2022-05-02 | GSE193636 | GEO
Project description:Germ cells are regulated by local microenvironments (niches), which secrete instructive cues. Conserved developmental signaling molecules act as niche-derived regulatory factors, yet other types of niche signals remain to be identified. Single-cell RNA-sequencing of sexual planarians revealed niche cells expressing a non-ribosomal peptide synthetase (nrps). Inhibiting nrps led to loss of female reproductive organs and testis hyperplasia. Mass spectrometry detected the dipeptide β-alanyl-tryptamine (BATT), which is associated with reproductive system development and requires nrps and a monoamine-transmitter-synthetic enzyme (AADC) for its production. Exogenous BATT rescued the reproductive defects after nrps or aadc inhibition, restoring fertility. Thus, a non-ribosomal, monoamine-derived peptide provided by niche cells acts as a critical signal to trigger planarian reproductive development. These findings reveal an unexpected function for monoamines in niche-germ cell signaling. Furthermore, given the recently reported role for BATT as a male-derived factor required for reproductive maturation of female schistosomes, these results have important implications for the evolution of parasitic flatworms and suggest a potential role for non-ribosomal peptides as signaling molecules in other organisms.
2024-06-06 | GSE267865 | GEO
Project description:Candida albicans can stochastically switch between two phenotypes, white and opaque. Opaque cells are the sexually competent form of C. albicans and therefore undergo efficient polarized growth and mating in the presence of pheromone. In contrast, white cells cannot mate, but are induced - under a specialized set of conditions - to form biofilms in response to pheromone. In this work, we compare the genetic regulation of such "pheromone-stimulated" biofilms with that of "conventional" C. albicans biofilms. In particular, we examined a network of six transcriptional regulators (Bcr1, Brg1, Efg1, Tec1, Ndt80, and Rob1) that mediate conventional biofilm formation for their potential roles in pheromone-stimulated biofilm formation. We show that four of the six transcription factors (Bcr1, Brg1, Rob1, and Tec1) promote formation of both conventional and pheromone-stimulated biofilms, indicating they play general roles in cell cohesion and biofilm development. In addition, we identify the master transcriptional regulator of pheromone-stimulated biofilms as C. albicans Cph1, ortholog of Saccharomyces cerevisiae Ste12. Cph1 regulates mating in C. albicans opaque cells, and here we show that Cph1 is also essential for pheromone-stimulated biofilm formation in white cells. In contrast, Cph1 is dispensable for the formation of conventional biofilms. The regulation of pheromone- stimulated biofilm formation was further investigated by transcriptional profiling and genetic analyses. These studies identified 206 genes that are induced by pheromone signaling during biofilm formation. One of these genes, HGC1, is shown to be required for both conventional and pheromone-stimulated biofilm formation. Taken together, these observations compare and contrast the regulation of conventional and pheromone-stimulated biofilm formation in C. albicans, and demonstrate that Cph1 is required for the latter, but not the former.
2013-02-22 | GSE44449 | GEO
Project description:Whole genome expression profile comparing MGAS315 treated with XIP pheromone versus vehicle-treated cells Interpretations are described further in the manuscript to be submitted: authors Mashburn-Warren, Morrison, and Federle. Title: The Cryptic Competence Pathway in Streptococcus pyogenes is Controlled by a Peptide Pheromone. A two chip study using total RNA recovered from three separate cultures of Streptococcus pyogenes MGAS315, each treated with either XIP pheromone or with vehicle; RNA preparation of cultures receiving same type of treatment were pooled using equivalent amounts of RNA from each culture. RNA pools were fluorescently labeled and hybridized to arrays designed to the S. pyogenes NZ131 genome.
2012-05-31 | E-GEOD-37974 | biostudies-arrayexpress