Project description:Ivory is a highly prized material in many cultures since it can be carved into intricated designs and have a highly polished surface. Due to its popularity, the animals from which ivory can be sourced have started to come under threat. Identification of the ivory species is not only important for compliance with the Convention on International Trade in Endangered Species of Wild Fauna and Flora (CITES), it can also provide important information about the context in which the work was created. In this work, we have developed a minimally invasive workflow to remove minimal amounts of material from precious objects, and, using high-resolution mass spectrometry-based proteomics, identified the taxonomy of several ivory and bone objects from the collection of The Metropolitan Museum of Art dating from as early as 4000 B.C. We built an inhouse proteomic databases of underrepresented species based on exemplars obtained from the Mammology American Museum of Natural History collection and proposed alternative data analysis workflows for rare samples containing sparse and inconsistently preserved organic material. This is a first application demonstrating extensive and accurate ivory species identification using proteomics to unlock sequence uncertainties, e.g. Leu/Ile-discrimination.
Project description:It remains challenging to study the viability and metabolic activity of microorganisms buried in ancient permafrost. We coupled aspartic acid racemization assay with metaproteomics to constraint the microbial activity of indigenous microbial community entrappped in permafrost sediment over 100 kyr ago.
Project description:Resistant Tiassale (Ivory Coast) strain compared to the lab susceptible N'Gousso. Tiassale was exposed to deltamethrin and the survivors were left for 48 hours prior to the array being performed.
Project description:Resistant M'Be (Ivory Coast) strain compared to the lab susceptible N'Gousso. M'Be was exposed to deltamethrin and the survivors were left for 48 hours prior to the array being performed.
Project description:Resistant Bouake (Ivory Coast) strain compared to the lab susceptible N'Gousso. Bouake was exposed to deltamethrin and the survivors were left for 48 hours prior to the array being performed.
Project description:Examination of aging in social insects. Differential gene expression between old and young individuals of four castes of the termite Macrotermes bellicosus. Minor workers, major workers, kings, queens. Collected in January 2015 in Ivory Coast.
