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RDNA Array Length is a Major Determinant of Replicative Lifespan in Budding Yeast - RNAseq

ABSTRACT: rDNA Array Length is a Major Determinant of Replicative Lifespan in Budding Yeast - RNAseq

PROVIDER: PRJNA796837 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR17601507_1.fastq.gz	Fastqsanger.gz
	SRR17601507_2.fastq.gz	Fastqsanger.gz
	SRR17601508_1.fastq.gz	Fastqsanger.gz
	SRR17601508_2.fastq.gz	Fastqsanger.gz
	SRR17601509_1.fastq.gz	Fastqsanger.gz

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rDNA Array Length is a Major Determinant of Replicative Lifespan in Budding Yeast - ATACseq

Project description:In this study we perform ATAC-seq of budding yeast strains with variable copy number of the rRNA genes (rDNA CN). We aim to better understand the relationship between DNA accessibility and replicative lifespan in strains with variable rDNA CN.

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rDNA Array Length is a Major Determinant of Replicative Lifespan in Budding Yeast.

Project description:rDNA Array Length is a Major Determinant of Replicative Lifespan in Budding Yeast.

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rDNA Array Length is a Major Determinant of Replicative Lifespan in Budding Yeast - ATACseq

Project description:rDNA Array Length is a Major Determinant of Replicative Lifespan in Budding Yeast - ATACseq

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rDNA Array Length is a Major Determinant of Replicative Lifespan in Budding Yeast - RNAseq

Project description:In this study we perform RNA-seq of budding yeast strains with variable copy number of the rRNA genes (rDNA CN) to assess the relationship between rDNA CN and gene expression. RNA-seq was performed on samples both with and without polyA selection (for all transcripts and for rRNA transcripts, respectively).

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rDNA array length is a major determinant of replicative lifespan in budding yeast.

Project description:The complex processes and interactions that regulate aging and determine lifespan are not fully defined for any organism. Here, taking advantage of recent technological advances in studying aging in budding yeast, we discovered a previously unappreciated relationship between the number of copies of the ribosomal RNA gene present in its chromosomal array and replicative lifespan (RLS). Specifically, the chromosomal ribosomal DNA (rDNA) copy number (rDNA CN) positively correlated with RLS and this interaction explained over 70% of variability in RLS among a series of wild-type strains. In strains with low rDNA CN, SIR2 expression was attenuated and extrachromosomal rDNA circle (ERC) accumulation was increased, leading to shorter lifespan. Suppressing ERC formation by deletion of FOB1 eliminated the relationship between rDNA CN and RLS. These data suggest that previously identified rDNA CN regulatory mechanisms limit lifespan. Importantly, the RLSs of reported lifespan-enhancing mutations were significantly impacted by rDNA CN, suggesting that changes in rDNA CN might explain the magnitude of some of those reported effects. We propose that because rDNA CN is modulated by environmental, genetic, and stochastic factors, considering rDNA CN is a prerequisite for accurate interpretation of lifespan data.

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rDNA Array Length is a Major Determinant of Replicative Lifespan in Budding Yeast.

Project description:This SuperSeries is composed of the SubSeries listed below.

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Yeast transcriptome profiling in replicative ageing

Project description:A crucial step towards understanding the mechanisms underlying aging is to obtain an integrated account of the molecular changes during aging. To address this, we mapped the yeast (S. cerevisiae) transcriptome during the replicative lifespan of budding yeast using novel culture and computational methods.

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