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Bacillus

ABSTRACT: Bacillus cereus group isolates from reptiles

PROVIDER: PRJNA799608 | ENA |

REPOSITORIES: ENA

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			Action	DRS
		Other
	SRR18728986_1.fastq.gz	Fastqsanger.gz
	SRR18728986_2.fastq.gz	Fastqsanger.gz
	SRR18728987_1.fastq.gz	Fastqsanger.gz
	SRR18728987_2.fastq.gz	Fastqsanger.gz

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Project description:The goal of this study is the discovery of (a) meaningful phylogenomic relationships among members of this B. cereus/B. anthracis group, and (b) reliable gene-phenotype associations, e.g. recognition of links between genomic traits and the ability of certain strains to cause various forms of disease. We also tried to elucidate genome evolution aspects that may lead to the emergence of variants that are capable (or have the potential) of causing anthrax-like disease. This large-scale comparative genomics approach is unprecedented for this taxonomic group. Dr. A. Hoffmaster (CDC) provided the PFGRC with 73 B. cereus and B. anthracis isolates from the CDC culture collection. Of these, 27 were isolated from patients with severe or systemic disease; ten isolates of this group were obtained from patients (welding factory workers) with anthrax-like disease or from the environment near their workplace. Another set of 26 represented isolates from food-born illnesses. Of the 26 gastrointestinal disease isolates (GIDI), 10 were obtained from patients with diarrhea, whereas another set of 10 had been shown to harbor the emetic (vomit) toxin gene by PCR. The rest of the group consisted of 20 isolates with various phenotypes. All strains were screened for their genomic content using the B. cereus/B. anthracis species microarray.

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