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LncRNA OIP5-AS1-directed miR-7 degradation promotes MYMX production during human myogenesis

ABSTRACT: LncRNA OIP5-AS1-directed miR-7 degradation promotes MYMX production during human myogenesis

PROVIDER: PRJNA837209 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR19167685_1.fastq.gz	Fastqsanger.gz
	SRR19167685_2.fastq.gz	Fastqsanger.gz
	SRR19167686_1.fastq.gz	Fastqsanger.gz
	SRR19167686_2.fastq.gz	Fastqsanger.gz
	SRR19167687_1.fastq.gz	Fastqsanger.gz

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LncRNA OIP5-AS1-directed miR-7 degradation promotes MYMX production during human myogenesis

Project description:The goals of this study are to compare differentially expressed RNAs during OIP5-AS1:MIR-7 TSB treated myoblasts and control myoblasts during myogenesis and further investigate functional regulatory network of those RNAs in myogenesis in vitro and in vivo.

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LncRNA OIP5-AS1-directed miR-7 degradation promotes MYMX production during human myogenesis.

Project description:Long noncoding RNAs (lncRNAs) and microRNAs (miRNAs) modulate gene expression programs in physiology and disease. Here, we report a noncoding RNA regulatory network that modulates myoblast fusion into multinucleated myotubes, a process that occurs during muscle development and muscle regeneration after injury. In early stages of human myogenesis, the levels of lncRNA OIP5-AS1 increased, while the levels of miR-7 decreased. Moreover, OIP5-AS1 bound and induced miR-7 decay via target RNA-directed miRNA decay; accordingly, loss of OIP5-AS1 attenuated, while antagonizing miR-7 accelerated, myotube formation. We found that the OIP5-AS1-mediated miR-7 degradation promoted myoblast fusion, as it derepressed the miR-7 target MYMX mRNA, which encodes the fusogenic protein myomixer (MYMX). Remarkably, an oligonucleotide site blocker interfered with the OIP5-AS1-directed miR-7 degradation, allowing miR-7 to accumulate, lowering MYMX production and suppressing myotube formation. These results highlight a mechanism whereby lncRNA OIP5-AS1-mediated miR-7 decay promotes myotube formation by stimulating a myogenic fusion program.

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LncRNA OIP5-AS1-directed miR-7 degradation promotes MYMX production during human myogenesis

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Effect of OIP5-AS1:MIR-7 TSB on myogenesis

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Identification of interacting proteins of lncRNA HIF1α-AS1

Project description:LncRNA Hypoxia-inducible factor 1α-antisense 1 (HIF1α-AS1) is located on the antisense strand of the important Hypoxia-inducible factor 1α (HIF1α) gene, but being transcribed in antisense direction along the HIF1α promoter. Here we used the 3’end biotinylated HIF1a-AS1 RNA and a control RNA for RNA Pulldown and searched for interacting proteins in nuclear extracts of human umbilical vein endothelial cells (HUVEC).

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HHIP-AS1 lncRNA promotes tumorigenicity in human SHH-driven brain tumors

Project description:Long non-coding RNAs (lncRNAs) comprise a diverse class of gene expression regulators with emerging roles in many biological processes including cancer. Here we show that the expression of the lncRNA Hedgehog Interacting Protein Antisense 1 (HHIP-AS1) is a hallmark feature of human SHH-driven tumors. Importantly, loss of HHIP-AS1 leads to reduced tumor growth in SHH-driven tumors in vitro and in vivo. Our results demonstrate the power of cross-entity transcriptome-wide comparisons to identify novel epigenetic–regulatory lncRNA circuitries underlying human cancers.

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