Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Enterococcus faecium EnGen0172 strain:VAN 222

ABSTRACT: Enterococcus faecium EnGen0172 strain:VAN 222 Genome sequencing and assembly

PROVIDER: PRJNA88363 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	AIUX01.dat.gz	Other
	AIUX01.fasta.gz	Fasta.gz
	AIUX01.master.dat	Other
	SRR530483_1.fastq.gz	Fastqsanger.gz
	SRR530483_2.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 12

Similar Datasets

Enterococcus faecium EnGen0171 strain:VAN 219

Project description:Enterococcus faecium EnGen0171 strain:VAN 219 Genome sequencing and assembly

| PRJNA88361 | ENA

Enterococcus faecium EnGen0371 strain:VAN 327

Project description:Enterococcus faecium EnGen0371 strain:VAN 327 Genome sequencing and assembly

| PRJNA157799 | ENA

Enterococcus faecium EnGen0372 strain:VAN 476

Project description:Enterococcus faecium EnGen0372 strain:VAN 476 Genome sequencing and assembly

| PRJNA157797 | ENA

Enterococcus faecium EnGen0173 strain:VAN 332

Project description:Enterococcus faecium EnGen0173 strain:VAN 332 Genome sequencing and assembly

| PRJNA88365 | ENA

Enterococcus faecium EnGen0176 strain:VAN 345

Project description:Enterococcus faecium EnGen0176 strain:VAN 345 Genome sequencing and assembly

| PRJNA88371 | ENA

Enterococcus faecium EnGen0175 strain:VAN 342

Project description:Enterococcus faecium EnGen0175 strain:VAN 342 Genome sequencing and assembly

| PRJNA88369 | ENA

Enterococcus faecium EnGen0174 strain:VAN 335

Project description:Enterococcus faecium EnGen0174 strain:VAN 335 Genome sequencing and assembly

| PRJNA88367 | ENA

Transcriptome mapping of Enterococcus faecalis and Enterococcus faecium

Project description:The enterococci comprise a genus of 49 low-GC content Gram-positive commensal species within the Firmicutes phylum that are known to occupy diverse habitats, notably the gastrointestinal core microbiota of nearly every phylum, including human. Of particular clinical relevance are two rogue species of enterococci, Enterococcus faecalis and the distantly related Enterococcus faecium, standing among the nefarious multi-drug resistant and hospital-acquired pathogens. Despite increasing evidence for RNA-based regulation in the enterococci, including regulation of virulence factors, their transcriptome structure and arsenal of regulatory small sRNAs (sRNAs) are not thoroughly understood. Using dRNA-seq, we have mapped at single-nucleotide resolution the primary transcriptomes of E. faecalis V583 and E. faecium AUS0004. We identified 2517 and 2771 transcription start sites (TSS) in E. faecalis and E. faecium, respectively. Based on the identified TSS, we created a global map of s70 promoter motifs. We also revealed features of 5’ and 3’UTRs across the genomes. The transcriptome maps also predicted 150 and 128 sRNA candidates in E. faecalis and E. faecium, respectively, some of which have been identified in previous studies and many of which are new. Finally, we validated several of the predicted sRNAs by Northern Blot in biologically relevant conditions. Comprehensive TSS mapping of two representative strains will provide a valuable resource for the continued development of RNA biology in the Enterococci.

2020-11-25 | GSE115009 | GEO