Dataset Information

Exploring Non-Canonical Terpene Space With a Heterologous Lepidopteran Mevalonate Pathway

ABSTRACT: Lepidoptera (butterflies and moths) make the six-carbon compounds homoisopentenyl pyrophosphate (HIPP) and homodimethylallyl pyrophosphate (HDMAPP) that are incorporated into sixteen, seventeen and eighteen carbon farnesyl pyrophosphate (FPP) analogues. In this work we heterologously expressed the lepidopteran modified mevalonate pathway, a propionyl-CoA ligase, and terpene cyclases in E. coli to produce several novel terpenes containing sixteen carbons. Changing the terpene cyclase generated different novel terpene product profiles. To further validate the new compounds we confirmed 13C propionate was incorporated, and that the masses and fragmentation patterns were consistent with novel sixteen carbon terpenes by GC-QTOF. Based on the available FPP analogues lepidoptera produce, this approach should greatly expand the reachable biochemical space with applications in areas where terpenes have traditionally found uses.

ORGANISM(S): Escherichia Coli

SUBMITTER: Chris Petzold

PROVIDER: PXD012697 | panorama | Tue Oct 29 00:00:00 GMT 2019

REPOSITORIES: PanoramaPublic

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Publications

Mevalonate Pathway Promiscuity Enables Noncanonical Terpene Production.

Eiben Christopher B CB de Rond Tristan T Bloszies Clayton C Gin Jennifer J Chiniquy Jennifer J Baidoo Edward E K EEK Petzold Christopher J CJ Hillson Nathan J NJ Fiehn Oliver O Keasling Jay D JD

ACS synthetic biology 20191002 10

Lepidoptera (butterflies and moths) make the six-carbon compounds homoisopentenyl pyrophosphate (HIPP) and homodimethylallyl pyrophosphate (HDMAPP) that are incorporated into 16, 17, and 18 carbon farnesyl pyrophosphate (FPP) analogues. In this work we heterologously expressed the lepidopteran modified mevalonate pathway, a propionyl-CoA ligase, and terpene cyclases in <i>E. coli</i> to produce several novel terpenes containing 16 carbons. Changing the terpene cyclase generated different novel t ...[more]

PMID: 31576747

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Dataset Information

Exploring Non-Canonical Terpene Space With a Heterologous Lepidopteran Mevalonate Pathway

Publications

Mevalonate Pathway Promiscuity Enables Noncanonical Terpene Production.

Similar Datasets

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