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Phosphoproteomic Effects of Acute Depletion of PP2A Regulatory Subunit Cdc55

ABSTRACT: Protein phosphatase regulatory subunits are increasingly recognized as promising drug targets. In the absence of an existing drug, inducible degradation provides a means of predicting candidate targets. We here employed auxin-inducible degradation of S. cerevisiae PP2A regulatory subunit Cdc55 in combination with quantitative phosphoproteomics. A prevalence of hyperphosphorylated phosphopeptides and enrichment of proteins containing the PP2A consensus sequence indicates that the approach successfully identified direct PP2A-Cdc55 targets. PRM follow up of DDA-results confirmed that vacuolar amino acid transporters are among the proteins most strongly affected by Cdc55 depletion.

ORGANISM(S): Saccharomyces Cerevisiae

SUBMITTER: Michael Plank

PROVIDER: PXD019534 | panorama | Wed Jan 27 00:00:00 GMT 2021

REPOSITORIES: PanoramaPublic

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Phosphoproteomic Effects of Acute Depletion of PP2A Regulatory Subunit Cdc55.

Plank Michael M Berti Marina M Loewith Robbie R

Proteomics 20201013 1

Protein phosphatase regulatory subunits are increasingly recognized as promising drug targets. In the absence of an existing drug, inducible degradation provides a means of predicting candidate targets. Here auxin-inducible degradation of Saccharomyces cerevisiae PP2A regulatory subunit Cdc55 in combination with quantitative phosphoproteomics is employed. A prevalence of hyperphosphorylated phosphopeptides indicates that the approach successfully identified direct PP2A<sup>Cdc55</sup> targets. P ...[more]

PMID: 32970932

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