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Phosphoproteomic Effects of Acute Depletion of PP2A Regulatory Subunit Cdc55

ABSTRACT: Protein phosphatase regulatory subunits are increasingly recognized as promising drug targets. In the absence of an existing drug, inducible degradation provides a means of predicting candidate targets. We here employed auxin-inducible degradation of S. cerevisiae PP2A regulatory subunit Cdc55 in combination with quantitative phosphoproteomics. A prevalence of hyperphosphorylated phosphopeptides and enrichment of proteins containing the PP2A consensus sequence indicates that the approach successfully identified direct PP2ACdc55 targets.

INSTRUMENT(S): Orbitrap Fusion

ORGANISM(S): Saccharomyces Cerevisiae (baker's Yeast)

SUBMITTER: Michael Plank

LAB HEAD: Robbie Joseph Loewith

PROVIDER: PXD019481 | Pride | 2020-10-15

REPOSITORIES: Pride

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			Action	DRS
	20190926_06_auxMock1_1ul.raw	Raw
	20190926_08_MockAux3_1ul.raw	Raw
	20190926_10_auxMock2_1ul.raw	Raw
	20190926_12_MockAux4_1ul.raw	Raw
	20190926_16_MockAux4_1ul.raw	Raw

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Phosphoproteomic Effects of Acute Depletion of PP2A Regulatory Subunit Cdc55.

Plank Michael M Berti Marina M Loewith Robbie R

Proteomics 20201013 1

Protein phosphatase regulatory subunits are increasingly recognized as promising drug targets. In the absence of an existing drug, inducible degradation provides a means of predicting candidate targets. Here auxin-inducible degradation of Saccharomyces cerevisiae PP2A regulatory subunit Cdc55 in combination with quantitative phosphoproteomics is employed. A prevalence of hyperphosphorylated phosphopeptides indicates that the approach successfully identified direct PP2A<sup>Cdc55</sup> targets. P ...[more]

PMID: 32970932

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