Ontology highlight
ABSTRACT:
ORGANISM(S): Escherichia Coli
SUBMITTER: David Foschepoth
PROVIDER: PXD020877 | panorama | Mon Sep 06 00:00:00 BST 2021
REPOSITORIES: PanoramaPublic
Doerr Anne A Foschepoth David D Forster Anthony C AC Danelon Christophe C
Scientific reports 20210121 1
The Protein synthesis Using Recombinant Elements (PURE) system enables transcription and translation of a DNA template from purified components. Therefore, the PURE system-catalyzed generation of RNAs and proteins constituting the PURE system itself represents a major challenge toward a self-replicating minimal cell. In this work, we show that all translation factors (except elongation factor Tu) and 20 aminoacyl-tRNA synthetases can be expressed in the PURE system from a single plasmid encoding ...[more]