Dataset Information

Prediction and experimental validation of a new salinity-responsive cis-regulatory element (CRE) in tilapia

ABSTRACT: Quantitative DIA proteomics in combination with the transcription inhibitor actinomycin D was performed on the tilapia OmB cell line to identify proteins that are upregulated by transcriptional regulation during hyperosmotic stress. This analysis revealed proteins that are transcriptionally up-regulated by hyperosmolality in these cells. The promoter regions of these proteins were compared and a novel hyperosmolality-induced cis-regulatory element (CRE) and corresponding transcription factor candidate were identified. The CRE was experimentlly validated by site-directed mutagenesis in combination with reporter assays.

ORGANISM(S): Oreochromis Mossambicus

SUBMITTER: Dietmar Kültz

PROVIDER: PXD032181 | panorama | Sun Sep 25 00:00:00 BST 2022

REPOSITORIES: PanoramaPublic

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Publications

Prediction and Experimental Validation of a New Salinity-Responsive Cis-Regulatory Element (CRE) in a Tilapia Cell Line.

Kim Chanhee C Wang Xiaodan X Kültz Dietmar D

Life (Basel, Switzerland) 20220525 6

Transcriptional regulation is a major mechanism by which organisms integrate gene x environment interactions. It can be achieved by coordinated interplay between cis-regulatory elements (CREs) and transcription factors (TFs). Euryhaline tilapia (<i>Oreochromis mossambicus</i>) tolerate a wide range of salinity and thus are an appropriate model to examine transcriptional regulatory mechanisms during salinity stress in fish. Quantitative proteomics in combination with the transcription inhibitor a ...[more]

PMID: 35743818

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Dataset Information

Prediction and experimental validation of a new salinity-responsive cis-regulatory element (CRE) in tilapia

Publications

Prediction and Experimental Validation of a New Salinity-Responsive Cis-Regulatory Element (CRE) in a Tilapia Cell Line.

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