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Omics score: 0

4Skin_iPS_BioReplica01

ABSTRACT: Hs iPS_vs_HES - 4Skin_iPS_BioReplica01

OTHER RELATED OMICS DATASETS IN: PXD000134PRJNA136559PRJNA142175PRJNA142177

INSTRUMENT(S): LTQ Orbitrap Velos

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Others

SUBMITTER: Munoz J, Low TY, Kok YJ, Chin A, Frese CK, Ding V, Choo A, Heck AJ

PROVIDER: PAe003659 | PeptideAtlas | 2011-12-31

REPOSITORIES: PeptideAtlas

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			Action	DRS
	PAe003659_7172_tandem.params	Other
	PAe003659_README	Other
	PAe003659_Search_Results_7172_201211161313.properties	Other
	PAe003659_Search_Results_7172_201211161313.tar.gz	Other
	PAe003659_mzML_201211161132.properties	Other

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Publications

The quantitative proteomes of human-induced pluripotent stem cells and embryonic stem cells.

Munoz Javier J Low Teck Y TY Kok Yee J YJ Chin Angela A Frese Christian K CK Ding Vanessa V Choo Andre A Heck Albert J R AJ

Molecular systems biology 20111122

Assessing relevant molecular differences between human-induced pluripotent stem cells (hiPSCs) and human embryonic stem cells (hESCs) is important, given that such differences may impact their potential therapeutic use. Controversy surrounds recent gene expression studies comparing hiPSCs and hESCs. Here, we present an in-depth quantitative mass spectrometry-based analysis of hESCs, two different hiPSCs and their precursor fibroblast cell lines. Our comparisons confirmed the high similarity of h ...[more]

PMID: 22108792

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Acute to prolonged lif cycle proteomic profiling of heat shock SGs

Project description:Time-resolved proteomic profiling of SGs from cultured human U2OS cells over a prolonged heat-shock (HS) and a successive recovery period, sampling at the 6 time points of HS exposure (10, 20, 30, 60, 120, 180 min), as well as 2 time points of recovery (5, 10 min). From the sampled cells, we prepared SG samples using a differential centrifugation plus endogenous G3BP1 immunoprecipitation protocol. Peptides were subsequently TMT-labeled and analyzed by quantitative LC-MS/MS. In G3BP1 dataset, rep1-rep3, HS-10, 20, 30, 60, 120, 180, re5, re10, SG-ls, G3BP1-IP samples were labeled with TMT-126, 127N, 127C, 128N, 128C,129N, 129C, 130N, 130C, 131 label Reagents, respectively; G3BP1_HS0 was a dataset created to integrate a non-heat shock control (HS-0) into the G3BP1 dataset, contains HS0, HS10, and HS60, and the results was integrated into the G3BP1 dataset using the ComBat processing, HS-0_rep1, HS-0_rep2, HS-0_rep3, HS-10_rep1, HS-10_rep2, HS-10_rep3, HS-60_rep1, HS-60_rep2, HS-60_rep3 samples were labeled with TMT-126, 127N, 127C, 128N, 128C,129N, 129C, 130N, 130C label Reagents, respectively. In CAPRIN1 dataset, rep1-rep3, HS-0, 10, 20, 30, 60, 120, 180, re5, re10 samples were labeled with TMT-126, 127N, 127C, 128N, 128C,129N, 129C, 130N, 130C label Reagents, respectively.

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Specific 3-O-sulfated heparan sulfate domains regulate salivary gland basement membrane metabolism and epithelial differentiation [male]

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