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Mm_astrocyte_WCL

ABSTRACT: In-depth proteomic analysis of secretome and whole cells of mouse astrocyte

REANALYSIS of: PXD000501

INSTRUMENT(S): QExactive

ORGANISM(S): Mus Musculus

SUBMITTER:

PROVIDER: PAe005211 | PeptideAtlas | 2014-12-31

REPOSITORIES: PeptideAtlas

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Proteomic analysis of mouse astrocytes and their secretome by a combination of FASP and StageTip-based, high pH, reversed-phase fractionation.

Han Dohyun D Jin Jonghwa J Woo Jongmin J Min Hophil H Kim Youngsoo Y

Proteomics 20140528 13-14

Astrocytes are the most abundant cells in the CNS, but their function remains largely unknown. Characterization of the whole-cell proteome and secretome in astrocytes would facilitate the study of their functions in various neurodegenerative diseases and astrocyte-neuron communication. To build a reference proteome, we established a C8-D1A astrocyte proteome to a depth of 7265 unique protein groups using a novel strategy that combined two-step digestion, filter-aided sample preparation, StageTip ...[more]

PMID: 24753479

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