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Hs_Hela_Tyr_Mitosis

ABSTRACT: HeLa S3 cells, Tyr phosphorylation, double thymidine block and nocodazole arrest

REANALYSIS of: PXD000612

INSTRUMENT(S): QExactive

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Others

SUBMITTER: Mario Oroshi

PROVIDER: PAe005270 | PeptideAtlas | 2014-12-31

REPOSITORIES: PeptideAtlas

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			Action	DRS
	PAe005270_10293_tandem.params	Other
	PAe005270_README	Other
	PAe005270_Search_Results_10293_201512312008.properties	Other
	PAe005270_Search_Results_10293_201512312008.tar.gz	Other
	PAe005270_mzXML_201512312007.properties	Other

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Publications

Ultradeep human phosphoproteome reveals a distinct regulatory nature of Tyr and Ser/Thr-based signaling.

Sharma Kirti K D'Souza Rochelle C J RC Tyanova Stefka S Schaab Christoph C Wiśniewski Jacek R JR Cox Jürgen J Mann Matthias M

Cell reports 20140821 5

Regulatory protein phosphorylation controls normal and pathophysiological signaling in eukaryotic cells. Despite great advances in mass-spectrometry-based proteomics, the extent, localization, and site-specific stoichiometry of this posttranslational modification (PTM) are unknown. Here, we develop a stringent experimental and computational workflow, capable of mapping more than 50,000 distinct phosphorylated peptides in a single human cancer cell line. We detected more than three-quarters of ce ...[more]

PMID: 25159151

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Transcription profiling of cell cycle in HeLa S3 cells, thymidine-nocodazole lock

Project description:HeLa S3 cells were synchronized in mitosis by arresting first in S phase with thymidine followed by arrest in mitosis with Nocodazole as described in Whitfield et al(2002)Mol.Biol.Cell. This experiment set contains the complete set of 43K arrays for the thymidine-nocodazole synchronization (Thy-Noc).

2005-09-22 | E-SMDB-904 | biostudies-arrayexpress

Cell division cycle in a human cancer cell line (HeLa)

Project description:This experiment set contains the complete set of raw data for Whitfield et al. (2002)Mol.Biol.Cell. HeLa S3 cells were synchronized by three different methods (double thymidine block, thymidine-nocodazole block or mitotic shake-off)in five independent time courses. mRNA from the first and second double thymidine block experiments were converted to cDNA by standard methods using an oligo-dT/random hexamer mix and hybridized to 24K arrays; mRNA from the third double thymidine and thymidine-nocodazole block experiments were converted to cDNA using olido-dT alone and hybridized to 43K arrays; total RNA from the mitotic shake-off experiment was first amplified using a modified eberwine protocol, aRNA converted to cDNA by reverse transcription with random hexamer and hybridized to 43K arrays. Set of arrays organized by shared biological context, such as organism, tumors types, processes, etc. Keywords: Logical Set

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Cell division cycle in a human cancer cell line (HeLa)

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cdr2 siRNA knockdown during passage through mitosis: HeLa cells, Rat1 wild type and c-myc null cells

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Transcription profiling of cell cycle in HeLa S3 cells, double thymidine block, rxperiment 2

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Identification of the cell cycle specific proximal interactors of PCDH7

Project description:We used proximity dependent biotin identification (BioID) method to determine cell cycle specific interaction partners of PCDH7. HeLa S3 cells were synchronized to interphase and mitosis using double tymidine block and double thymidine block followed by S-Trityl-L-cysteine (STC) treatment respectively. Candidate interactors were isolated using streptavidin affinity purification and identified using LC-MS/MS analysis.

2023-02-27 | PXD029246 | Pride

Hs_Hela_Mitosis

Project description:HeLa S3 cells, double thymidine block and nocodazole arrest

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MeRIPseq of m6A in HeLa synchonized cells with total RNA

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