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Hs_Hela_Tyr_Pervanadate

ABSTRACT: HeLa S3 cells, Tyr phosphorylation, thymidine block and nocodazole arrest, treated with pervanadate

REANALYSIS of: PXD000612

INSTRUMENT(S): QExactive

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Others

SUBMITTER: Mario Oroshi

PROVIDER: PAe005300 | PeptideAtlas | 2014-12-31

REPOSITORIES: PeptideAtlas

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			Action	DRS
	PAe005300_10347_tandem.params	Other
	PAe005300_README	Other
	PAe005300_Search_Results_10347_201512312023.properties	Other
	PAe005300_Search_Results_10347_201512312023.tar.gz	Other
	PAe005300_mzXML_201512312008.properties	Other

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1 - 5 of 8

Publications

Ultradeep human phosphoproteome reveals a distinct regulatory nature of Tyr and Ser/Thr-based signaling.

Sharma Kirti K D'Souza Rochelle C J RC Tyanova Stefka S Schaab Christoph C Wiśniewski Jacek R JR Cox Jürgen J Mann Matthias M

Cell reports 20140821 5

Regulatory protein phosphorylation controls normal and pathophysiological signaling in eukaryotic cells. Despite great advances in mass-spectrometry-based proteomics, the extent, localization, and site-specific stoichiometry of this posttranslational modification (PTM) are unknown. Here, we develop a stringent experimental and computational workflow, capable of mapping more than 50,000 distinct phosphorylated peptides in a single human cancer cell line. We detected more than three-quarters of ce ...[more]

PMID: 25159151

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Project description:HeLa S3 cells were synchronized in mitosis by arresting first in S phase with thymidine followed by arrest in mitosis with Nocodazole as described in Whitfield et al(2002)Mol.Biol.Cell. This experiment set contains the complete set of 43K arrays for the thymidine-nocodazole synchronization (Thy-Noc).

2005-09-22 | E-SMDB-904 | biostudies-arrayexpress

Cell division cycle in a human cancer cell line (HeLa)

Project description:This experiment set contains the complete set of raw data for Whitfield et al. (2002)Mol.Biol.Cell. HeLa S3 cells were synchronized by three different methods (double thymidine block, thymidine-nocodazole block or mitotic shake-off)in five independent time courses. mRNA from the first and second double thymidine block experiments were converted to cDNA by standard methods using an oligo-dT/random hexamer mix and hybridized to 24K arrays; mRNA from the third double thymidine and thymidine-nocodazole block experiments were converted to cDNA using olido-dT alone and hybridized to 43K arrays; total RNA from the mitotic shake-off experiment was first amplified using a modified eberwine protocol, aRNA converted to cDNA by reverse transcription with random hexamer and hybridized to 43K arrays. Set of arrays organized by shared biological context, such as organism, tumors types, processes, etc. Keywords: Logical Set

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