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Hs_C-HPP_Testis_Tricine-SDS-PAGE

ABSTRACT: CN-CHPP 2015 Testis, Tricine-SDS-PAGE

REANALYSIS of: PXD002179

INSTRUMENT(S): LTQ Orbitrap Velos

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Male Reproductive System

SUBMITTER: Zhang Y, Li Q, Wu F, Zhou R, Qi Y, Su N, Chen L, Xu S, Jiang T, Zhang C, Cheng G, Chen X, Kong D, Wang Y, Zhang T, Zi J, Wei W, Gao Y, Zhen B, Xiong Z, Wu S, Yang P, Wang Q, Wen B, He F, Xu P, Liu S

PROVIDER: PAe005333 | PeptideAtlas | 2015-12-31

REPOSITORIES: PeptideAtlas

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Tissue-Based Proteogenomics Reveals that Human Testis Endows Plentiful Missing Proteins.

Zhang Yao Y Li Qidan Q Wu Feilin F Zhou Ruo R Qi Yingzi Y Su Na N Chen Lingsheng L Xu Shaohang S Jiang Tao T Zhang Chengpu C Cheng Gang G Chen Xinguo X Kong Degang D Wang Yujia Y Zhang Tao T Zi Jin J Wei Wei W Gao Yuan Y Zhen Bei B Xiong Zhi Z Wu Songfeng S Yang Pengyuan P Wang Quanhui Q Wen Bo B He Fuchu F Xu Ping P Liu Siqi S

Journal of proteome research 20150901 9

Investigations of missing proteins (MPs) are being endorsed by many bioanalytical strategies. We proposed that proteogenomics of testis tissue was a feasible approach to identify more MPs because testis tissues have higher gene expression levels. Here we combined proteomics and transcriptomics to survey gene expression in human testis tissues from three post-mortem individuals. Proteins were extracted and separated with glycine- and tricine-SDS-PAGE. A total of 9597 protein groups were identifie ...[more]

PMID: 26282447

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