Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Quantitative proteome analysis of human blister fluids using 18O labeling

ABSTRACT: Not available

INSTRUMENT(S): LTQ Orbitrap, instrument model

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Serum

SUBMITTER: Joost Gouw

PROVIDER: PRD000059 | Pride | 2008-05-14

REPOSITORIES: pride

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	PRIDE_Exp_Complete_Ac_3640.pride.mgf.gz	Mgf
	PRIDE_Exp_Complete_Ac_3640.pride.mztab.gz	Mztab
	PRIDE_Exp_Complete_Ac_3640.xml.gz	Xml
	PRIDE_Exp_Complete_Ac_3641.pride.mgf.gz	Mgf
	PRIDE_Exp_Complete_Ac_3641.pride.mztab.gz	Mztab

Items per page:

1 - 5 of 171

Similar Datasets

Quantitative analysis of human plasma in vivo lipopolysaccharide administration using ¹?O/¹?O Labeling and AMT

Project description:Not available

2012-07-26 | PRD000478 | Pride

Quantitative proteome analysis of human plasma following in vivo lipopolysaccharide administration using 16O/18O labeling and the accurate mass and time tag approach.

Project description:Identification of novel diagnostic or therapeutic biomarkers from human blood plasma would benefit significantly from quantitative measurements of the proteome constituents over a range of physiological conditions. Herein we describe an initial demonstration of proteome-wide quantitative analysis of human plasma. The approach utilizes postdigestion trypsin-catalyzed 16O/18O peptide labeling, two-dimensional LC-FTICR mass spectrometry, and the accurate mass and time (AMT) tag strategy to identify and quantify peptides/proteins from complex samples. A peptide accurate mass and LC elution time AMT tag data base was initially generated using MS/MS following extensive multidimensional LC separations to provide the basis for subsequent peptide identifications. The AMT tag data base contains >8,000 putative identified peptides, providing 938 confident plasma protein identifications. The quantitative approach was applied without depletion of high abundance proteins for comparative analyses of plasma samples from an individual prior to and 9 h after lipopolysaccharide (LPS) administration. Accurate quantification of changes in protein abundance was demonstrated by both 1:1 labeling of control plasma and the comparison between the plasma samples following LPS administration. A total of 429 distinct plasma proteins were quantified from the comparative analyses, and the protein abundances for 25 proteins, including several known inflammatory response mediators, were observed to change significantly following LPS administration.

| S-EPMC1829297 | biostudies-literature

Quantitative erythrocyte membrane proteome analysis with Blue-Native SDS PAGE

Project description:Not available

2011-04-15 | PRD000092 | Pride

Analysis of Trypanosome flagellar proteome using a combined electron transfer/collisionally activated dissociation strategy

Project description:Not available

2010-04-29 | PRD000806 | Pride

Non-ionic Detergent Phase Extraction for the Quantitative Proteomic Analysis of Heart Membranes Proteins using Label-Free LC-MS

Project description:Not available

2008-08-21 | PRD000043 | Pride

Comparative analysis of the human and mouse placental transcriptome and proteome

Project description:Not available

2012-07-26 | PRD000474 | Pride

High-accuracy proteome maps of human body fluids.

Project description:The proteomes most likely to contain clinically useful disease biomarkers are those of human body fluids. Three recent large-scale proteomic analyses of tears, urine and seminal plasma using the latest mass spectrometric technology will provide useful datasets for biomarker discovery.

| S-EPMC1794581 | biostudies-literature

Yarrowia lipolytica proteome - Proteome analysis

Project description:Not available

2007-05-04 | PRD000022 | Pride

Ustilago maydis proteome - Proteome analysis

Project description:Not available

2007-05-04 | PRD000761 | Pride

Ashbya gossypii proteome - Proteome analysis

Project description:Not available

2007-05-04 | PRD000759 | Pride