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Identification of ciliary neurotrophic factor receptor alpha as a mediator of neurotoxicity induced by extracellular alpha-synuclein

ABSTRACT: MES cells were treated with vehicle or 250 nM extracellular alpha-synuclein and plasma membrane proteins were labeled with biotin, followed by affinity purification. The biotinylated enriched membranous proteins were incubated with an alpha-synuclein peptide array membrane, washed extensively with TBS-T, followed by incubation with avidin-HRP. The positive spots were excised and the bound proteins were eluted. The elutions from consecutive spots were combined before trypsin digestion and analysis of peptides by LC-MS/MS.

INSTRUMENT(S): LCQ Deca, instrument model

ORGANISM(S): Mus Musculus X Rattus Norvegicus

TISSUE(S): Dopaminergic Neuron, Permanent Cell Line Cell

SUBMITTER: Jing Zhang

PROVIDER: PRD000155 | Pride | 2009-12-01

REPOSITORIES: Pride

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			Action	DRS
	PRIDE_Exp_Complete_Ac_10061.pride.mgf.gz	Mgf
	PRIDE_Exp_Complete_Ac_10061.xml.gz	Xml
	PRIDE_Exp_Complete_Ac_10062.pride.mgf.gz	Mgf
	PRIDE_Exp_Complete_Ac_10062.xml.gz	Xml
	PRIDE_Exp_Complete_Ac_10063.pride.mgf.gz	Mgf

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Project description:Alpha-synuclein is an abundant protein implicated in synaptic function and plasticity, but the molecular mechanism of its action is not understood. Missense mutations and gene duplication/triplication events result in Parkinson's disease, a neurodegenerative disorder of old age with impaired movement and emotion control. Here, we systematically investigated the striatal as well as the cerebellar transcriptome profile of alpha-synuclein-deficient mice via a genome-wide microarray survey in order to gain hypothesis-free molecular insights into the physiological function of alpha-synuclein. A genotype-dependent, specific and strong downregulation of forkhead box P1 (Foxp1) transcript levels was observed in all brain regions from postnatal age until old age and could be validated by qPCR. In view of the co-localization and heterodimer formation of FOXP1 with FOXP2, a transcription factor with a well established role for vocalization, and the reported regulation of both alpha-synuclein and FOXP2 expression during avian song learning, we performed a detailed assessment of mouse movements and vocalizations in the postnatal period. While there was no difference in isolation-induced behavioral activity in these animals, the alpha-synuclein-deficient mice exhibited an increased production of isolation-induced ultrasonic vocalizations (USVs). This phenotype might also reflect the reduced expression of the anxiety-related GABA-A receptor subunit gamma 2 (Gabrg2) we observed. Taken together, we identified an early behavioral consequence of alpha-synuclein deficiency and accompanying molecular changes, which supports the notion that the neural connectivity of sound or emotion control systems is affected. Factorial design comparing SNCA knock-out mice with wild type littermates in two different tissues (striatum, cerebellum) at two different timepoints (6 and 21 month)

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