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24hrs IGF-1 - Temporal proteomic analysis of IGF-1R signalling in MCF-7 breast adenocarcinoma cells

ABSTRACT: 24 hr stimulated MCF-7 cells (0,6,12,24 h) labelled with iTRAQ reagents

INSTRUMENT(S): instrument model, Waters instrument model

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: Patrick Murphy

PROVIDER: PRD000178 | Pride | 2010-04-29

REPOSITORIES: pride

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			Action	DRS
	PRIDE_Exp_Complete_Ac_10639.pride.mgf.gz	Mgf
	PRIDE_Exp_Complete_Ac_10639.pride.mztab.gz	Mztab
	PRIDE_Exp_Complete_Ac_10639.xml.gz	Xml
	PRIDE_Exp_Complete_Ac_10885.pride.mgf.gz	Mgf
	PRIDE_Exp_Complete_Ac_10885.pride.mztab.gz	Mztab

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Temporal proteomic analysis of IGF-1R signalling in MCF-7 breast adenocarcinoma cells.

Murphy J Patrick JP Pinto Devanand M DM

Proteomics 20100501 9

Dysregulation of the insulin-like growth factor 1 receptor signalling network is implicated in tumour growth and resistance to chemotherapy. We explored proteomic changes resulting from insulin-like growth factor 1 stimulation of MCF-7 adenocarcinoma cells as a function of time. Quantitative analysis using iTRAQ reagents and 2-D LC-MS/MS analysis of three biological replicates resulted in the identification of 899 proteins (p<or=0.05) with an estimated mean false-positive rate of 2.6%. Quantitat ...[more]

PMID: 20213678

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Effects of IGF 1 on primary breast fibroblasts, MCF-7 breast cancer cells and CCL-171 fibroblasts

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Expression profiling of breast cancer cell lines MCF-7 and MCF-7R4

Project description:Human breast cancer cell line MCF-7 is usually sensitive to chemotherapy drug BMS-554417, an insulin receptor (IR) and insulin-like growth factor receptor (IGFR) inhibitor. However, through step-wise increase in BMS-554417 doses in culture media, we were able able to screen and select a single MCF-7 clone that is BMS-554417 resistant. It is cross resistant to BMS-536924. This new line of MCF-7 cells was named as MCF-7R4. The transcriptome profiling of both MCF-7 and MCF-7R4 was performed using Affymetrix HG-U133 plus2.0 GeneChip arrays.

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