Dataset Information

Cold stress in rice - Rice seedlings were exposed to low temperature and leaves were harvested after 0, 48, 72 and 96 hours. Proteins were analysed using label-free quantitative proteomics.

ABSTRACT: Rice seedlings were exposed to low temperature and leaves were harvested after 0, 48, 72 and 96 hours. Proteins were analysed using label-free quantitative proteomics.

INSTRUMENT(S): instrument model, AB SCIEX instrument model, LTQ XL

ORGANISM(S): Oryza Sativa (rice)

SUBMITTER: Karlie Neilson

PROVIDER: PRD000361 | Pride | 2011-03-03

REPOSITORIES: Pride

ACCESS DATA

Dataset's files

Source:

			Action	DRS
	PRIDE_Exp_Complete_Ac_15854.pride.mgf.gz	Mgf
	PRIDE_Exp_Complete_Ac_15854.xml.gz	Xml
	PRIDE_Exp_Complete_Ac_15855.pride.mgf.gz	Mgf
	PRIDE_Exp_Complete_Ac_15855.xml.gz	Xml
	PRIDE_Exp_Complete_Ac_15856.pride.mgf.gz	Mgf

Items per page:

1 - 5 of 60

Similar Datasets

Project description:BackgroundRice is one of the most important cereals consumed worldwide. Two major abiotic factors affecting rice plants in different growth stages are flooding stress and cold stress. These abiotic stresses can take place independently or simultaneously and significantly affect rice plants during germination and seedling growth. Fortunately, a wide array of phenotypic responses conferring flooding stress and chilling stress tolerance exist within the rice germplasm, indicating the presence of different molecular mechanisms underlying tolerance to these stresses. Understanding these differences may assist in developing improved rice cultivars having higher tolerance to both stresses. In this study, we conducted a comparative global gene expression analysis of two rice genotypes with contrasting phenotypes under cold stress, anaerobic stress, and combined cold and anaerobic stress during germination.ResultsThe differential gene expression analysis revealed that 5571 differentially expressed genes (DEGs), 7206 DEGs, and 13279 DEGs were identified under anaerobic stress, cold stress, and combined stress, respectively. Genes involved in the carbohydrate metabolic process, glucosyltransferase activity, regulation of nitrogen compound metabolic process, protein metabolic process, lipid metabolic process, cellular nitrogen compound biosynthetic process, lipid biosynthetic process, and a microtubule-based process were enriched across all stresses. Notably, the common Gene Ontology (GO) analysis identified three hub genes, namely Os08g0176800 (similar to mRNA-associated protein mrnp 41), Os11g0454200 (dehydrin), and OS10g0505900 (expressed protein).ConclusionA large number of differentially expressed genes were identified under anaerobic, cold conditions during germination and the combination of the two stress conditions in rice. These results will assist in the identification of promising candidate genes for possible manipulation toward rice crops that are more tolerant under flooding and cold during germination, both independently and concurrently.

Dataset Information

Cold stress in rice - Rice seedlings were exposed to low temperature and leaves were harvested after 0, 48, 72 and 96 hours. Proteins were analysed using label-free quantitative proteomics.

Dataset's files

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets