Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

RNA-seq profiling of Tetep (resistant) and HP2216 (susceptible) rice lines at 0, 48, 72, and 96 hours post infection after M. oryzae inoculation in panicle

ABSTRACT: Purpose: The aim of this study was to compare transcriptome profiling (RNA-seq) of rice panicle tissue from resistant and suceptible lines at different time intervals infected with M.oryzae. The results were validated using Semi-quantitative PCR. Methods: Panicles from resistant and suceptible rice lines were inoculated with suspension having double distill water, Tween-20 without spores for 0 hour and same suspension with spores was used to inoculate for 48, 72 and 96 hour samples. After collection, samples were frozen on liquid nitrogen, and RNA was extracted using Spectrum™ Plant Total RNA Kit (Sigma). RNA libraries were prepared for sequencing using standard Illumina protocols. Results: Differential Expressed Genes were identified, comparing transcriptome of Control 0 hour vs 48, 72 and 96 hour post infection. Where we get 1070, 2383 and 1080 total significant genes at 48, 72, and 96 hour post infection respectively in HP2216. And, 1564, 1433 and 2263 total significant genes at 48, 72, and 96 hour post infection respectively in Tetep.

ORGANISM(S): Oryza sativa Indica Group

PROVIDER: GSE136672 | GEO | 2021/07/29

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

N-Tera2 differentiated human neuronal model: Mock infected vs HCoV-OC43 infected

Project description:Transcriptional profiling of N-Tera2 differentiated human neuronal cells, comparing control uninfected cells to HCoV-OC43 infected cells at 24, 48 and 72 hour post-infection Keywords: Cell response to viral infection

2008-12-11 | GSE13879 | GEO

Cold stress in rice - Rice seedlings were exposed to low temperature and leaves were harvested after 0, 48, 72 and 96 hours. Proteins were analysed using label-free quantitative proteomics.

Project description:Rice seedlings were exposed to low temperature and leaves were harvested after 0, 48, 72 and 96 hours. Proteins were analysed using label-free quantitative proteomics.

2011-03-03 | PRD000361 | Pride

C. elegans gene expression in response to Salmonella enterica infection and recovery by Tetracycline treatment

2014-07-26 | GSE54212 | GEO

Exosomal microRNA (miRNA) expression in Blonchoalveolar lavage fluid (BALF) collected from mice infected with influenza virus and inoculated with poly(I:C).

Project description:To clarify the profile of in BALF exosome collected from mice infected with influenza virus, we infected 100000 pfu of A/Puerto Rico/8/1934 (PR8) strain. BALF was collected at 24, 48, and 72 hour post infection (hpi). For comparison of the profile of the miRNA in BALF exosome induced by innate immune response, we also intranasally inoculated mice with 50 μg of poly(I:C) and collected BALF at 72 hour post inoculation. We found that some miRNAs were common to both influenza virus infectiona and poly(I:C) inoculation, suggesting that exosomal miRNAs in BALF may function in the innate immune response to virus infection.

2018-06-08 | GSE107107 | GEO

Structurally Diverse PFAS Produce Unique Transcriptomic Changes Linked to Developmental Toxicity in Zebrafish

Project description:Transcriptomic data for 48 hours post-fertilization (hpf) wildtype zebrafish developmentally exposed to 15 PFAS, and for 48, 72, and 96 hpf wildtype zebrafish developmentally exposed to nafion byproduct 2.

2024-07-20 | GSE252004 | GEO

Transcription profiling of mouse naive CD8+ T-cell differentiation during a primary immune response

Project description:Title: Gene Expression analysis of naive CD8+ T-cell differentiation during a primary immune response Description: C57BL/6 wild type mice are administered GK1.5 (depleting anti CD4 antibody) or isotope control by intraperitoneal injection at 3 and 1 day pre-infection, then every 36-48 hour post infection. 24 hours pre-infection, splenocytes from F5 RAG -/- mice are stained with CFSE and 10^7 splenocytes are transfered to recipient C57BL/6 mice by intravenous injection. 24 hours laters, recipient mice are infected with 10^7 PFU of a recombinant Vaccinia virus expressing NP366-74. At timepoints 0, 12, 24, 48, 72 and 96 splenocytes are obtained and FACS sorted to obtain CD8+ T-cells.

2005-09-29 | E-MIMR-38 | biostudies-arrayexpress

SHAE003: SARS-CoV, SARS-dORF6 and SARS-BatSRBD infection of HAE cultures.

Project description:HAE cultures were infected with SARS-CoV, SARS-ddORF6 or SARS-BatSRBD and were directly compared to A/CA/04/2009 H1N1 influenza-infected cultures. Cell samples were collected at various hours post-infection for analysis. Time Points = 0, 12, 24, 36, 48, 60, 72, 84 and 96 hrs post-infection for SARS-CoV. Time Points = 0, 24, 48, 60, 72, 84 and 96 hrs post-infection forSARS-ddORF6 and SARS-BatSRBD. Time Points = 0, 6, 12, 18, 24, 36 and 48 hrs post-infection for H1N1. Done in triplicate/quadruplicate for RNA Triplicates/quadruplicates are defined as 3/4 different wells, plated at the same time and using the same cell stock for all replicates. Time matched mocks done in triplicate from same cell stock as rest of samples. Culture medium (the same as what the virus stock is in) will be used for the mock infections. Infection was done at an MOI of 2.

2013-07-31 | E-GEOD-47961 | biostudies-arrayexpress

Cytosolic proteome of Staphylococcus aureus HG001 post internalization in bronchial epithelial cells - human dataset

Project description:The data set consists of 32 cytosolic samples of human bronchial epithelial cells that were collected during infection with S. aureus. The data include 8 (0h, 1h, 2.5 h, 6.5 h, 24 h, 48 h, 72 h, and 96 h post infection) time points that were collected out of four biological replicates. DIA data and the established human cell line specific spectral library are provided.

| MSV000082923 | MassIVE

Expression data from susceptible rice cultivar innoculated with Magnaporthe oryzae isolate (Mo-ei-11)

Project description:The molecular mechanisms underlying infection of rice host system with compatible M. oryzae isolates at 12, 24, 48 and 72 hours post innoculation (hpi) are investigated in the study. In addition, the identification of highly commonly upregulated genes by both isolates of M. oryzae may be useful candidates for M.oryzae inducible promoter characterization We used microarrays to detail the global programme of gene expression underlying M. oryzae infection during compatible host-pathogen interaction

2018-02-28 | GSE95394 | GEO

C. elegans gene expression in response to Salmonella enterica infection and recovery by Tetracycline treatment

Project description:(Part 1) Gene expression profiles of C. elegans in response to a 120 hour S. enterica infection. Synchronized larval stage 1 (L1) animals were exposed to S. enterica SL1344 for 36, 72, 96, or 120 hours. As an uninfected control, synchronized L1 animals were exposed to E. coli OP50 for 36 hours. (Part 2) Gene expression profiles of C. elegans in response to Tetracycline-mediated recovery from 72 hour and 96 hour S. enterica infections. Synchronized L1 animals were exposed to S. enterica SL1344 for 72 hours and then shifted to E. coli HT115 plus Tetracycline plates for 24 hours to resolve the infection. Synchronized L1 animals were exposed to S. enterica SL1344 for 96 hours and then shifted to E. coli HT115 plus Tetracycline plates for 24 hours to resolve the infection. Samples include 36 hours on OP50 (36hOP), 36 hours on SL1344 (36hSL), 72 hours on SL1344 (72hSL), 96 hours on SL1344 (96hSL), 120 hours on SL1344 (120hSL), 72 hours on SL1344 plus 24 hours on HT115-Tet (96hHT), 96 hours on SL1344 plus 24 hours on HT115-Tet (120hHT). Duplicate biological samples were included for each timepoint. 14 total samples. Agilent C. elegans expression microarray (GPL14144).

2014-07-26 | E-GEOD-54212 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data