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Biosynthesis of cyanogenic defence compunds in plants and insects

ABSTRACT: peaklists for identification of P450s and UGTs from different Zyagaena tissue

INSTRUMENT(S): LTQ Orbitrap, instrument model

ORGANISM(S): Zygaena Filipendulae

SUBMITTER: Jonas Borch

PROVIDER: PRD000404 | Pride | 2011-03-31

REPOSITORIES: Pride

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			Action	DRS
	PRIDE_Exp_Complete_Ac_16842.pride.mgf.gz	Mgf
	PRIDE_Exp_Complete_Ac_16842.xml.gz	Xml
	PRIDE_Exp_Complete_Ac_16843.pride.mgf.gz	Mgf
	PRIDE_Exp_Complete_Ac_16843.xml.gz	Xml
	PRIDE_Exp_Complete_Ac_16844.pride.mgf.gz	Mgf

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1 - 5 of 18

Publications

Convergent evolution in biosynthesis of cyanogenic defence compounds in plants and insects.

Jensen Niels Bjerg NB Zagrobelny Mika M Hjernø Karin K Olsen Carl Erik CE Houghton-Larsen Jens J Borch Jonas J Møller Birger Lindberg BL Bak Søren S

Nature communications 20110101

For more than 420 million years, plants, insects and their predators have co-evolved based on a chemical arms race including deployment of refined chemical defence systems by each player. Cyanogenic glucosides are produced by numerous plants and by some specialized insects and serve an important role as defence compounds in these intimate interactions. Burnet moth larvae are able to sequester cyanogenic glucosides from their food plant as well as to carry out de novo biosynthesis. Here we show t ...[more]

PMID: 21505429

Similar Datasets

Convergent evolution in biosynthesis of cyanogenic defence compounds in plants and insects.

Project description:For more than 420 million years, plants, insects and their predators have co-evolved based on a chemical arms race including deployment of refined chemical defence systems by each player. Cyanogenic glucosides are produced by numerous plants and by some specialized insects and serve an important role as defence compounds in these intimate interactions. Burnet moth larvae are able to sequester cyanogenic glucosides from their food plant as well as to carry out de novo biosynthesis. Here we show that three genes (CYP405A2, CYP332A3 and UGT33A1) encode the entire biosynthetic pathway of cyanogenic glucosides in the Burnet moth Zygaena filipendulae. In both plants and insects, convergent evolution has led to two multifunctional P450 enzymes each catalysing unusual reactions and a glucosyl-transferase acting in sequence to catalyse cyanogenic glucoside formation. Thus, plants and insects have independently found a way to package a cyanide time bomb to fend off herbivores and predators.

| S-EPMC4354137 | biostudies-literature

Biotransformation of efavirenz and proteomic analysis of P450s and UGTs in human, mouse, and macaque brain-derived in vitro systems

Project description:Antiretroviral drugs such as efavirenz (EFV) are essential to combat HIV infection in the brain, but little is known about how these drugs are metabolized locally. In this study, the cytochrome P450 (P450) and UDP-glucuronosyltransferase (UGT)-dependent metabolism of EFV was probed in brain microsomes from mice, cynomolgus macaques, and humans as well as primary neural cells from C57BL/6N mice. Utilizing ultra-high performance liquid chromatography high resolution mass spectrometry (uHPLC-HRMS), the formation of 8-hydroxyefavirenz (8-OHEFV) from EFV and the glucuronidation of P450-dependent metabolites 8-OHEFV and 8,14-dihydroxyefavirenz (8,14-diOHEFV) was observed in brain microsomes from all three species. The direct glucuronidation of EFV, however, was only detected in the cynomolgus macaque brain microsomes. In brain cell typesprimary neural cells treated with EFV, microglia formed 8-OHEFV only while glucuronidation was detected in cortical neurons and astrocytes treated with P450-dependent metabolites of EFV. Untargeted and targeted proteomics experiments were used to determine which P450s and UGTs were present in the brain microsomes. Eleven P450s and 11 UGTs were detected in the human brain microsomes, whilewith 10 P450s and 15 UGTs were identified in the mouse brain microsomes and 17 and 6 P450s and UGTs, respectively, observed in the macaque brain microsomes. For many of these enzymes, this was the first time they have been noted at the protein level in the brain microsomes. This study indicates the potential for the brain to play a contributing role in the local metabolism of EFV, and the related pharmacological and toxicological consequences.metabolism to contribute to pharmacological and toxicological EFV outcomes in brain.

2022-10-24 | MSV000090576 | MassIVE

Small RNA sequencing in Arabidopsis plants

Project description:Identification of microRNA expressed in Arabidopsis plants grown at different ambient CO2 concentrations and different ambient temperatures

2013-12-22 | GSE36934 | GEO

Identification of microRNAs specifically expressed in HCV-associated hepatocellular carcinoma

Project description:Background: Several studies have investigated the association of miRNAs with hepatocellular carcinoma (HCC) but the data are not univocal. Methods: We performed a microarray study of miRNAs in hepatitis C virus (HCV)-associated HCC and other liver diseases and healthy conditions. Results and Conclusions: The simultaneous comparison of different liver diseases and normal livers allowed the identification of 18 miRNAs exclusively expressed in HCV-associated HCC, with sensitivity and specificity values of diagnostic-grade. A total number of 76 liver specimens obtained from 43 patients were analyzed: 26 liver specimens obtained from 10 patients with HCV-associated HCC, including 9 specimens from the tumor area (HCC) and 17 specimens from the surrounding non-tumorous tissue affected by cirrhosis (HCC-CIR); 18 specimens from 10 patients with HCV-associated cirrhosis without HCC (CIR); 13 specimens from 4 patients with HBV-associated acute liver failure (ALF); 12 specimens from 12 liver donors (LD); and 7 from normal liver of 7 subjects who underwent hepatic resection for liver angioma (NL).

2013-02-20 | E-GEOD-40744 | biostudies-arrayexpress

Time-serialized expression profile of MeJA induced Andrographis paniculata leaves

Project description:To shed light on the biosynthesis mechanisms of these diterpenes, we performed the first high-throughput RNA-seq for A. paniculata leaves with time-serialized MeJA treatments. The transcriptomic approach not only defined a comprehensive transcriptome (comprised of 20,629 highly expressed genes), but also profiled time-serialized gene expression patterns with 4,463 up-regulated genes and 6,517 down-regulated genes responding to MeJA induction. These up-regulated genes significantly enriched in biological processes like stress responses, metabolic pathways, and secondary metabolites biosynthesis, especially terpene/terpenoid metabolic process. Consistent with the gradual accumulation of andrographolide, the expression of most genes involved in MEP pathway showed progressive increase. In the hypothetical terpenoid metabolism pathway, ApCPS2, which was putatively involved in andrographolide synthesis, dramatically increased its expression. Among other co-regulated genes, 154 genes encode cytochrome P450s (CYP450s); 42 genes were predicted as UDP glycosyltransferases (UGTs); and 363 genes belong to transcription factors.

2018-12-31 | GSE83775 | GEO

Broad-range metalloprotease profiling in plants uncovers immunity provided by defence-related metalloenzyme

Project description:Plants encode for >100 metalloproteases representing >19 different protein families. Tools to study this large and diverse class of proteases have not yet been introduced into plant science. Here, we used hydroxamate-based photoaffinity probes to explore plant proteomes for metalloproteases. We detected labeling of 23 metalloproteases in leaf extracts of the model plant Arabidopsis thaliana that belong to eight unrelated metalloprotease families and localize to different subcellular compartments. We identified several chloroplast localized FtsH proteases; vacuolar aspartyl aminopeptidase DAP1; peroxisomal metalloprotease PMX16; and many cytosolic metalloproteases. We also identified non-proteolytic metallohydrolases involved the release of auxin and in the urea cycle. Studies on tobacco plant Nicotiana benthamiana infected with the bacterial plant pathogen Pseudomonas syringae uncovered PRp27, a secreted protein with anticipated metalloprotease activity. PRp27 depletion increases susceptibility, whilst its overexpression increases resistance, dependent on the presence of active site residues. Collectively, these studies uncover the strength of hydroxamate-based probes to study metalloenzymes in plants

2022-08-12 | PXD032265 | Pride

Characterization of individual mouse cerebrospinal fluid proteomes

Project description:Through non-terminal CSF extractions in C57Bl/6 mice and high-resolution liquid chromatography-mass spectrometry analysis of individual murine samples, we report the most comprehensive proteome characterization of individual murine CSF to date. Utilizing stringent protein inclusion criteria that required the identification of at least two unique peptides (1% false discovery rate at the peptide level) we identified a total of 566 unique proteins, including 128 proteins from three individual CSF samples that have been previously identified in brain tissue.

2014-04-09 | PXD000248 | Pride

Vitamin B6 biosynthesis in higher plants.

Project description:Vitamin B6 is an essential metabolite in all organisms. It can act as a coenzyme for numerous metabolic enzymes and has recently been shown to be a potent antioxidant. Plants and microorganisms have a de novo biosynthetic pathway for vitamin B6, but animals must obtain it from dietary sources. In Escherichia coli, it is known that the vitamin is derived from deoxyxylulose 5-phosphate (an intermediate in the nonmevalonate pathway of isoprenoid biosynthesis) and 4-phosphohydroxy-l-threonine. It has been assumed that vitamin B6 is synthesized in the same way in plants, but this hypothesis has never been experimentally proven. Here, we show that, in plants, synthesis of the vitamin takes an entirely different route, which does not involve deoxyxylulose 5-phosphate but instead utilizes intermediates from the pentose phosphate pathway, i.e., ribose 5-phosphate or ribulose 5-phosphate, and from glycolysis, i.e., dihydroxyacetone phosphate or glyceraldehyde 3-phosphate. The revelation is based on the recent discovery that, in bacteria and fungi, a novel pathway is in place that involves two genes (PDX1 and PDX2), neither of which is homologous to any of those involved in the previously doctrined E. coli pathway. We demonstrate that Arabidopsis thaliana has two functional homologs of PDX1 and a single homolog of PDX2. Furthermore, and contrary to what was inferred previously, we show that the pathway appears to be cytosolic and is not localized to the plastid. Last, we report that the single PDX2 homolog is essential for plant viability.

| S-EPMC1224648 | biostudies-literature

Proteomics of TGF beta signaling

Project description:HaCaT cells were treated with TGF beta1 for different time points to follw protein and phosphoproteome changes on a temporal scale. Samples were analyzed using a shotgun proteomic approach on a Q exactive coupled to reverse phase HPLC. The measurements resulted in the identification of over 20,000 phopshorylation sites and over 6,000 protein expression profiles.

2014-07-01 | PXD000496 | Pride

Hijacking common mycorrhizal networks for herbivore-induced defence signal transfer between tomato plants.

Project description:Common mycorrhizal networks (CMNs) link multiple plants together. We hypothesized that CMNs can serve as an underground conduit for transferring herbivore-induced defence signals. We established CMN between two tomato plants in pots with mycorrhizal fungus Funneliformis mosseae, challenged a 'donor' plant with caterpillar Spodoptera litura, and investigated defence responses and insect resistance in neighbouring CMN-connected 'receiver' plants. After CMN establishment caterpillar infestation on 'donor' plant led to increased insect resistance and activities of putative defensive enzymes, induction of defence-related genes and activation of jasmonate (JA) pathway in the 'receiver' plant. However, use of a JA biosynthesis defective mutant spr2 as 'donor' plants resulted in no induction of defence responses and no change in insect resistance in 'receiver' plants, suggesting that JA signalling is required for CMN-mediated interplant communication. These results indicate that plants are able to hijack CMNs for herbivore-induced defence signal transfer and interplant defence communication.

| S-EPMC3904153 | biostudies-other

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