Dataset Information

Characterization of individual mouse cerebrospinal fluid proteomes

ABSTRACT: Through non-terminal CSF extractions in C57Bl/6 mice and high-resolution liquid chromatography-mass spectrometry analysis of individual murine samples, we report the most comprehensive proteome characterization of individual murine CSF to date. Utilizing stringent protein inclusion criteria that required the identification of at least two unique peptides (1% false discovery rate at the peptide level) we identified a total of 566 unique proteins, including 128 proteins from three individual CSF samples that have been previously identified in brain tissue.

REANALYSED by: PAe005205

INSTRUMENT(S): LTQ Orbitrap Velos

ORGANISM(S): Mus Musculus (mouse)

SUBMITTER: Matthew Monroe

PROVIDER: PXD000248 | Pride | 2014-04-09

REPOSITORIES: Pride

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			Action	DRS
	Mouse_CSF_1_2_2_TAngel_Frac-01_08Mar12_Tiger_12-01-15.mgf	Mgf
	Mouse_CSF_1_2_2_TAngel_Frac-01_08Mar12_Tiger_12-01-15.raw	Raw
	Mouse_CSF_1_2_2_TAngel_Frac-01_08Mar12_Tiger_12-01-15_msgfplus.mzid	Mzid
	Mouse_CSF_1_2_2_TAngel_Frac-02_08Mar12_Tiger_12-01-14.mgf	Mgf
	Mouse_CSF_1_2_2_TAngel_Frac-02_08Mar12_Tiger_12-01-14.raw	Raw

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Characterization of individual mouse cerebrospinal fluid proteomes.

Smith Jeffrey S JS Angel Thomas E TE Chavkin Charles C Orton Daniel J DJ Moore Ronald J RJ Smith Richard D RD

Proteomics 20140320 9

Analysis of cerebrospinal fluid (CSF) offers key insight into the status of the CNS. Characterization of murine CSF proteomes can provide a valuable resource for studying CNS injury and disease in animal models. However, the small volume of CSF in mice has thus far limited individual mouse proteome characterization. Through nonterminal CSF extractions in C57Bl/6 mice and high-resolution 2D-LC MS/MS analysis of individual murine samples, we report the most comprehensive proteome characterization ...[more]

PMID: 24677814

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