Dataset Information

Detection of SNO by cysTMT switch

ABSTRACT: HAPEC treated with SNO-Cys or control compounds

INSTRUMENT(S): instrument model, LTQ Velos

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: Christopher Murray

PROVIDER: PRD000410 | Pride | 2012-01-16

REPOSITORIES: pride

ACCESS DATA

Dataset's files

Source:

			Action	DRS
	PRIDE_Exp_Complete_Ac_16868.pride.mgf.gz	Mgf
	PRIDE_Exp_Complete_Ac_16868.pride.mztab.gz	Mztab
	PRIDE_Exp_Complete_Ac_16868.xml.gz	Xml

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Publications

Identification and quantification of S-nitrosylation by cysteine reactive tandem mass tag switch assay.

Murray Christopher I CI Uhrigshardt Helge H O'Meally Robert N RN Cole Robert N RN Van Eyk Jennifer E JE

Molecular & cellular proteomics : MCP 20111129 2

Redox-switches are critical cysteine thiols that are modified in response to changes in the cell's environment conferring a functional effect. S-nitrosylation (SNO) is emerging as an important modulator of these regulatory switches; however, much remains unknown about the nature of these specific cysteine residues and how oxidative signals are interpreted. Because of their labile nature, SNO-modifications are routinely detected using the biotin switch assay. Here, a new isotope coded cysteine th ...[more]

PMID: 22126794

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