Dataset Information

Network organization of the human exosomes

ABSTRACT: Exosomes, nano-sized bilayered proteolipids, play multiple roles in intercellular communication. Although many exosomal proteins have been identified, their functional interrelationships and the mechanisms of exosome biogenesis remain unknown. By interrogating proteomic data using systems approaches, we have created a human exosome network, which comprises 1491 interactions between 957 exosomal proteins. Moreover, physically and functionally interconnected protein complexes form functional modules involved in exosome biogenesis and functions. Specifically, we discovered that SRC signaling plays a major role in exosome biogenesis, and confirmed that inhibition of SRC kinase decreased the intracellular biogenesis and cell surface release of exosomes. Our study provides global insights into the cargo-sorting, biogenesis, and pathophysiological roles of these complex extracellular organelles.

INSTRUMENT(S): instrument model, LTQ

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Colorectal Cancer Cell Line

SUBMITTER: Yong Song Gho

PROVIDER: PRD000414 | Pride | 2012-02-09

REPOSITORIES: Pride

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Dataset's files

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			Action	DRS
	PRIDE_Exp_Complete_Ac_16880.pride.mgf.gz	Mgf
	PRIDE_Exp_Complete_Ac_16880.pride.mztab.gz	Mztab
	PRIDE_Exp_Complete_Ac_16880.xml.gz	Xml

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Publications

The protein interaction network of extracellular vesicles derived from human colorectal cancer cells.

Choi Dong-Sic DS Yang Jae-Seong JS Choi Eun-Jeong EJ Jang Su Chul SC Park Solip S Kim Oh Youn OY Hwang Daehee D Kim Kwang Pyo KP Kim Yoon-Keun YK Kim Sanguk S Gho Yong Song YS

Journal of proteome research 20120109 2

Various mammalian cells including tumor cells secrete extracellular vesicles (EVs), otherwise known as exosomes and microvesicles. EVs are nanosized bilayered proteolipids and play multiple roles in intercellular communication. Although many vesicular proteins have been identified, their functional interrelationships and the mechanisms of EV biogenesis remain unknown. By interrogating proteomic data using systems approaches, we have created a protein interaction network of human colorectal cance ...[more]

PMID: 22149170

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