Browse
Submit Data
Databases
API
Help

Dataset Information

216 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Vacuolar and tonoplast proteins from potato cv. Kuras

ABSTRACT: Proteomic analysis of vacuolar and tonoplast proteins from potato cv. Kuras

INSTRUMENT(S): Bruker Daltonics micrOTOF series, instrument model

ORGANISM(S): Solanum Tuberosum (potato)

SUBMITTER: Allan stensballe

PROVIDER: PRD000450 | Pride | 2011-09-03

REPOSITORIES: Pride

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	PRIDE_Exp_Complete_Ac_17707.pride.mgf.gz	Mgf
	PRIDE_Exp_Complete_Ac_17707.pride.mztab.gz	Mztab
	PRIDE_Exp_Complete_Ac_17707.xml.gz	Xml

Items per page:

1 - 3 of 3

Publications

Extensive post-translational processing of potato tuber storage proteins and vacuolar targeting.

Jørgensen Malene M Stensballe Allan A Welinder Karen Gjesing KG

The FEBS journal 20110922 21

Potato tuber storage proteins were obtained from vacuoles isolated from field-grown starch potato tubers cv. Kuras. Vacuole sap proteins fractionated by gel filtration were studied by mass spectrometric analyses of trypsin and chymotrypsin digestions. The tuber vacuole appears to be a typical protein storage vacuole absent of proteolytic and glycolytic enzymes. The major soluble storage proteins included 28 Kunitz protease inhibitors, nine protease inhibitors 1, eight protease inhibitors 2, two ...[more]

PMID: 21851554

Similar Datasets

Dynamics of tolerant potato-potato virus Y interaction

Project description:Time series response of potato cv. Désirée, which is tolerant to PVY infection, was analysed in both inoculated as well as upper non-inoculated leaves. Additionally, transgenic plants deficient in accumulation of salicylic acid (NahG- Désirée) were studied in the same setting.

2015-08-06 | GSE58593 | GEO

The vacuolar sugar transporter EARLY RESPONSE TO DEHYDRATION 6-LIKE4 regulates fructose signaling and plant growth

Project description:Regulation of intracellular sugar homeostasis is maintained by regulation of activities of sugar import and export proteins residing at the tonoplast. We show here that the EARLY RESPONSE TO DEHYDRATION6-LIKE4 protein, being the closest homolog to the proton/glucose symporter ERDL6, resides in the vacuolar membrane. Gene expression and subcellular fractionation studies indicated that ERDL4 was involved in fructose allocation across the tonoplast. Overexpression of ERDL4 increased total sugar levels in leaves, due to a concomitantly induced stimulation of TST2 expression, coding for the major vacuolar sugar loader. This conclusion is supported by the finding that tst1-2 knockout lines overexpressing ERDL4 lack increased cellular sugar levels. ERDL4 activity contributing to the coordination of cellular sugar homeostasis is also indicated by two further observations. Firstly, ERDL4 and TST genes exhibit an opposite regulation during a diurnal rhythm, and secondly, the ERDL4 gene is markedly expressed during cold acclimation representing a situation in which TST activity needs to be upregulated. Moreover, ERDL4-overexpressing plants show larger size of rosettes and roots, a delayed flowering time and increased total seed yield. Consistently, erdl4 knock-out plants show impaired cold acclimation and freezing tolerance along with reduced plant biomass. In summary, we show that modification of cytosolic fructose levels influences plant organ development and stress tolerance.

2023-01-15 | GSE222562 | GEO

MOA:Multiomics analysis of potato response to Potato virus Y (PVY) infection

2019-09-25 | PXD015221 | Pride

Potato secretome during Phytophthora infection

Project description:Secretome samples were taken at different time points during Phytophthora infection of potato leaves.

2014-06-24 | PXD000435 | Pride

Early response of potato plants to virus (PVY-NTN) inoculation

Project description:Potato virus YNTN (PVYNTN), causing potato tuber ring necrosis disease, dramatically lowers the quantity and the quality of the potato yield all over the world. The cultivar Igor is one of the most susceptible cultivars, developing severe disease symptoms on plants as well as on tubers. Finding genes differentially expressed in the early response to infection, when the host response is more defense- than infection- related, could improve our understanding of the potato - PVYNTN interaction. Differential gene expression in early response of potato cv. Igor plants to PVYNTN infection was studied using potato TIGR cDNA-microarrays. Expression was compared between mock inoculated and virus infected plants 12 hours after inoculation, in four biological replicates. Keywords: direct comparison

2008-03-21 | GSE10903 | GEO

The potato tuber mitochondrial proteome

Project description:The potato tuber mitochondrial proteome Three biological replicates, 40 gel sections each (120 files) MS raw files (.raw) and peptide search files [MASCOT (.msf), MSGFBD (.txt), PROLUCID (.sqt) and SEQUEST (_sequest.msf)] Mitochondria were isolated from dormant potato tubers (Solanum tuberosum) and proteins (0.25 mg) resolved by one-dimensional gel electrophoresis , sectioned into 40 equal segments, and tryptic peptides analyzed by liquid chromatography tandem mass spectrometry using an Orbitrap XL. Acquired MS/MS spectra were searched using different algorithms against the potato protein database (http://www.potatogenome.net). To estimate the protein false discovery rate (FDR), randomized sequences were combined with the forward database in a concatenate format. MASCOT server 2.3.01, SEQUEST, MS-GFDB and ProLuCID software were used to interpret the data set using very similar parameters. MASCOT and SEQUEST were used integrated within Proteome Discoverer 1.3 software package (Thermo Scientific). Raw files (Thermo) were converted to ms2 and mzXML files to run ProLuCID and MS-GFDB softwares. All search engines were configured to the following parameters: MW range between 200 and 2,000; 1,000 ppm of precursor ion tolerance; oxidation of methionine and asparagine deamidation as variable modifications and carbamidomethylation of cysteine as a static modification; trypsin; 2 allowed missed cleavages. After searches, the PSMs were filtered out at 5ppm of precursor ion tolerance achieving a false discovery rate (FDR) lower than 1% at the protein level for each biological replicate. Filtered data were uploaded on Protein Herder module within Compass package for protein grouping. At this step, all sets of indistinguishable proteins, which were assigned by the same peptides, are combined into protein groups.

2014-01-28 | PXD000149 | Pride

Time course study of phosphite treated potato leaflets

Project description:Time course study of potato leaflets (cv. Desiree) 3, 6, 11, 24 and 48 hours after phosphite treatment

2014-10-27 | E-MTAB-2243 | biostudies-arrayexpress

BABA treatment of the potato cultivar Desiree

Project description:Induced resistance study in potato. Experiment conducted on leaves from one cultivar (cv. Desiree) 48 hours after treatment with the inducing agent BABA.

2014-04-25 | E-MTAB-1545 | biostudies-arrayexpress

Phytophthora infestans infection of three potato cultivars

Project description:Time course study of potato leaves from three cultivars (cv. Desiree, Sarpo Mira, SW-1015) 6, 24 and 72 hours after P. infestans infection

2014-06-23 | E-MTAB-1515 | biostudies-arrayexpress

Dynamics of tolerant potato-potato virus Y interaction

2015-08-06 | E-GEOD-58593 | biostudies-arrayexpress