Dataset Information

Spiroplasma melliferum KC3 - Application of Spiroplasma melliferum proteogenomic profiling for the discovery of virulence factors and pathogenicity mechanisms in host-associated spiroplasmas

ABSTRACT: Not available

OTHER RELATED OMICS DATASETS IN: PRJNA32299

INSTRUMENT(S): instrument model

ORGANISM(S): Spiroplasma Melliferum Kc3 Spiroplasma Melliferum

SUBMITTER: Dmitry Alexeev

PROVIDER: PRD000548 | Pride | 2013-09-30

REPOSITORIES: Pride

ACCESS DATA

Dataset's files

Source:

			Action	DRS
	PRIDE_Exp_Complete_Ac_19037.pride.mgf.gz	Mgf
	PRIDE_Exp_Complete_Ac_19037.xml.gz	Xml
	PRIDE_Exp_Complete_Ac_19038.pride.mgf.gz	Mgf
	PRIDE_Exp_Complete_Ac_19038.xml.gz	Xml
	PRIDE_Exp_Complete_Ac_19039.pride.mgf.gz	Mgf

Items per page:

1 - 5 of 6

Publications

Application of Spiroplasma melliferum proteogenomic profiling for the discovery of virulence factors and pathogenicity mechanisms in host-associated spiroplasmas.

Alexeev Dmitry D Kostrjukova Elena E Aliper Alexander A Popenko Anna A Bazaleev Nikolay N Tyakht Alexander A Selezneva Oksana O Akopian Tatyana T Prichodko Elena E Kondratov Ilya I Chukin Mikhail M Demina Irina I Galyamina Maria M Kamashev Dmitri D Vanyushkina Anna A Ladygina Valentina V Levitskii Sergei S Lazarev Vasily V Govorun Vadim V

Journal of proteome research 20111216 1

To date, no genome of any of the species from the genus Spiroplasma has been completely sequenced. Long repetitive sequences similar to mobile units present a major obstacle for current genome sequencing technologies. Here, we report the assembly of the Spiroplasma melliferum KC3 genome into 4 contigs, followed by proteogenomic annotation and metabolic reconstruction based on the discovery of 521 expressed proteins and comprehensive metabolomic profiling. A systems approach allowed us to elucida ...[more]

PMID: 22129229

Similar Datasets

Project description:Outer membrane vesicles (OMVs) are important tools in bacterial virulence but their role in the pathogenesis of infections caused by enterohemorrhagic Escherichia coli (EHEC) O157, the leading cause of life-threatening hemolytic uremic syndrome, is poorly understood. Using proteomics, electron and confocal laser scanning microscopy, immunoblotting, and bioassays, we investigated OMVs secreted by EHEC O157 clinical isolates for virulence factors cargoes, interactions with pathogenetically relevant human cells, and mechanisms of cell injury. We demonstrate that O157 OMVs carry a cocktail of key virulence factors of EHEC O157 including Shiga toxin 2a (Stx2a), cytolethal distending toxin V (CdtV), EHEC hemolysin, and flagellin. The toxins are internalized by cells via dynamin-dependent endocytosis of OMVs and differentially separate from vesicles during intracellular trafficking. Stx2a and CdtV-B, the DNase-like CdtV subunit, separate from OMVs in early endosomes. Stx2a is trafficked, in association with its receptor globotriaosylceramide within detergent-resistant membranes, to the Golgi complex and the endoplasmic reticulum from where the catalytic Stx2a A1 fragment is translocated to the cytosol. CdtV-B is, after its retrograde transport to the endoplasmic reticulum, translocated to the nucleus to reach DNA. CdtV-A and CdtV-C subunits remain OMV-associated and are sorted with OMVs to lysosomes. EHEC hemolysin separates from OMVs in lysosomes and targets mitochondria. The OMV-delivered CdtV-B causes cellular DNA damage, which activates DNA damage responses leading to G2 cell cycle arrest. The arrested cells ultimately die of apoptosis induced by Stx2a and CdtV via caspase-9 activation. By demonstrating that naturally secreted EHEC O157 OMVs carry and deliver into cells a cocktail of biologically active virulence factors, thereby causing cell death, and by performing first comprehensive analysis of intracellular trafficking of OMVs and OMV-delivered virulence factors, we provide new insights into the pathogenesis of EHEC O157 infections. Our data have implications for considering O157 OMVs as vaccine candidates.