Ontology highlight
ABSTRACT:
INSTRUMENT(S): instrument model, LTQ
ORGANISM(S): Saccharomyces Cerevisiae (baker's Yeast)
SUBMITTER: Mitsuyoshi Ueda
PROVIDER: PRD000729 | Pride | 2012-07-20
REPOSITORIES: Pride
Miura Natsuko N Kirino Aya A Endo Satoshi S Morisaka Hironobu H Kuroda Kouichi K Takagi Masahiro M Ueda Mitsuyoshi M
Eukaryotic cell 20120629 8
Glycolytic enzymes are cytosolic proteins, but they also play important extracellular roles in cell-cell communication and infection. We used Saccharomyces cerevisiae to analyze the secretory pathway of some of these enzymes, including enolase, phosphoglucose isomerase, triose phosphate isomerase, and fructose 1,6-bisphosphate aldolase. Enolase, phosphoglucose isomerase, and an N-terminal 28-amino-acid-long fragment of enolase were secreted in a sec23-independent manner. The enhanced green fluor ...[more]