Dataset Information

Proteomics analysis of wound healing in porcine subcutaneous fatty tissue postmonopolar electrosurgery and harmonic blade incisions.

ABSTRACT: Two female pigs weighing 40-55 kg were used. Each animal had four 4-cm midline caudal abdominal incisions created with the test devices. The test devices were monopolar electrosurgery (Electrocautery Pencil, ConMed, Utica NY) and Harmonic blade (HK105, Ethicon Endo-Surgery, Cincinnati OH). Each animal had two Harmonic Blade incisions and two Electrosurgery incisions for a total of 8 incisions. The incisions were created by marking the site on the ventral aspect of the abdomen and identified by level from cranial to caudal, as incision A, B, C and D respectively. The incision was started with a scalpel blade through the skin (epidermis and dermis). Each incision was deepened through the subcutaneous tissues down to the linea alba using the Harmonic at power level 5 for cutting tissue and power level 3 for coagulating bleeders, or electrosurgery set at 40 Watts for cutting and Blend 2/Spray for coagulation. The linea alba was not incised. Closure of the incision was accomplished by apposing the skin with 3-0 nylon in a simple interrupted pattern. Dermabond was then applied to seal the epidermis. No bandages were applied to the incisions. After three days, incision sites were removed en bloc from the abdominal wall. The tissue block was sliced perpendicular to the surgically incision to create slabs 4-6 mm thick. The slab was trimmed to remove the skin from the top and muscle from the bottom of the sample. The side walls were trimmed to approximately 4 mm from the surgical incision leaving a rectangular block of subcutaneous fatty tissue. Four to six samples were taken from each incision. Animals were then euthanized. Samples for genomics and proteomics analysis were placed in sterile DNAse-, RNAse-, protein-free Eppendorf tubes, flash frozen in liquid nitrogen, held on dry ice throughout tissue collection, and then stored at -80 C. Tissue samples were also collected in 10% neutral buffered formalin for histopathology analysis.

OTHER RELATED OMICS DATASETS IN: PRJNA207344E-GEOD-47710

INSTRUMENT(S): LCQ Deca XP Plus

ORGANISM(S): Sus Scrofa Domesticus (domestic Pig)

SUBMITTER: Ken Pendarvis

PROVIDER: PXD000038 | Pride | 2014-04-15

REPOSITORIES: Pride

ACCESS DATA

Dataset's files

Source:

Items per page:

1 - 5 of 108

Publications

Ultrasonic incisions produce less inflammatory mediator response during early healing than electrosurgical incisions.

Nanduri Bindu B Pendarvis Ken K Shack Leslie A LA Kumar Ranjit R Clymer Jeffrey W JW Korvick Donna L DL Burgess Shane C SC

PloS one 20130918 9

As the use of laparoscopic surgery has become more widespread in recent years, the need has increased for minimally-invasive surgical devices that effectively cut and coagulate tissue with reduced tissue trauma. Although electrosurgery (ES) has been used for many generations, newly-developed ultrasonic devices (HARMONIC® Blade, HB) have been shown at a macroscopic level to offer better coagulation with less thermally-induced tissue damage. We sought to understand the differences between ES and H ...[more]

PMID: 24058457

Similar Datasets

Project description:Cursons2015 - Regulation of ERK-MAPK signaling in human epidermis Model comparing the abundance of phosphorylated MAPK signalling proteins and calcium signalling in the epidermis. This model is described in the article: Regulation of ERK-MAPK signaling in human epidermis. Cursons J, Gao J, Hurley DG, Print CG, Dunbar PR, Jacobs MD, Crampin EJ. BMC Syst Biol 2015; 9: 41 Abstract: The skin is largely comprised of keratinocytes within the interfollicular epidermis. Over approximately two weeks these cells differentiate and traverse the thickness of the skin. The stage of differentiation is therefore reflected in the positions of cells within the tissue, providing a convenient axis along which to study the signaling events that occur in situ during keratinocyte terminal differentiation, over this extended two-week timescale. The canonical ERK-MAPK signaling cascade (Raf-1, MEK-1/2 and ERK-1/2) has been implicated in controlling diverse cellular behaviors, including proliferation and differentiation. While the molecular interactions involved in signal transduction through this cascade have been well characterized in cell culture experiments, our understanding of how this sequence of events unfolds to determine cell fate within a homeostatic tissue environment has not been fully characterized.We measured the abundance of total and phosphorylated ERK-MAPK signaling proteins within interfollicular keratinocytes in transverse cross-sections of human epidermis using immunofluorescence microscopy. To investigate these data we developed a mathematical model of the signaling cascade using a normalized-Hill differential equation formalism.These data show coordinated variation in the abundance of phosphorylated ERK-MAPK components across the epidermis. Statistical analysis of these data shows that associations between phosphorylated ERK-MAPK components which correspond to canonical molecular interactions are dependent upon spatial position within the epidermis. The model demonstrates that the spatial profile of activation for ERK-MAPK signaling components across the epidermis may be maintained in a cell-autonomous fashion by an underlying spatial gradient in calcium signaling.Our data demonstrate an extended phospho-protein profile of ERK-MAPK signaling cascade components across the epidermis in situ, and statistical associations in these data indicate canonical ERK-MAPK interactions underlie this spatial profile of ERK-MAPK activation. Using mathematical modelling we have demonstrated that spatially varying calcium signaling components across the epidermis may be sufficient to maintain the spatial profile of ERK-MAPK signaling cascade components in a cell-autonomous manner. These findings may have significant implications for the wide range of cancer drugs which therapeutically target ERK-MAPK signaling components. This model is hosted on BioModels Database and identified by: BIOMD0000000659. To cite BioModels Database, please use: Chelliah V et al. BioModels: ten-year anniversary. Nucl. Acids Res. 2015, 43(Database issue):D542-8. To the extent possible under law, all copyright and related or neighbouring rights to this encoded model have been dedicated to the public domain worldwide. Please refer to CC0 Public Domain Dedication for more information.

			Action	DRS
	C1_01_DDF1.RAW	Raw
	C1_01_DDF1.mgf	Mgf
	C1_01_DDF2.RAW	Raw
	C1_01_DDF2.mgf	Mgf
	C1_01_DDF3.RAW	Raw