Dataset Information

GeLC-MS/MS analysis of cereal aphid saliva

ABSTRACT: Saliva was collected from c.40,000 diet reared aphids, pooled and concentrated. Samples were coming from 2 aphid species: Metopolophium dirhodum and Sitobion avenae. Proteins were fractionated using SDS PAGE and visible bands were subjected to in gel digestion. Tryptic peptides were analysised on a Thermo LTQ-FT. Protein identification from the MS/MS data was performed using the TurboSEQUEST algorithm in BioWorks v. 3.2 (Thermo Fisher Scientific) to correlate the data against ACYPIproteins v2.1, the official protein set of the pea aphid genome assembly (33291 predicted protein models; accessed November 2011) available at http://www.aphidbase.com/aphidbase/downloads. The following search parameters were used: precursor-ion mass tolerance of 1.5 Da, fragment ion tolerance of 1.0 Da with methionine oxidation and cysteine carboxyamidomethylation specified as differential modifications and a maximum of two missed cleavage sites allowed. Two filters were applied: XCorr vs. charge state (1, 2, 3 and 4 = 1.50, 2.00, 2.50 and 3.00 respectively) and peptide probability (p<0.001). Matches with multiple unique peptides and a cumulative XCorr >20 are reported. PLoS One accepted: Proteomic profiling of cereal aphid saliva reveals ubiquitous and adaptative secreted proteins. Rao S.A.K., Carolan, J.C. and Wilkinson, T.L.W.

INSTRUMENT(S): LTQ

ORGANISM(S): Metopolophium Dirhodum (rose Grain Aphid) Sitobion Avenae (english Grain Aphid)

SUBMITTER: James Carolan

LAB HEAD: James Carolan

PROVIDER: PXD000113 | Pride | 2013-04-09

REPOSITORIES: Pride

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Publications

Proteomic profiling of cereal aphid saliva reveals both ubiquitous and adaptive secreted proteins.

Rao Sohail A K SA Carolan James C JC Wilkinson Tom L TL

PloS one 20130227 2

The secreted salivary proteins from two cereal aphid species, Sitobion avenae and Metopolophium dirhodum, were collected from artificial diets and analysed by tandem mass spectrometry. Protein identification was performed by searching MS data against the official protein set from the current pea aphid (Acyrthosiphon pisum) genome assembly and revealed 12 and 7 proteins in the saliva of S. avenae and M. dirhodum, respectively. When combined with a comparable dataset from A. pisum, only three indi ...[more]

PMID: 23460852

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			Action	DRS
	MD_saliva_02.zip	Other
	Md_saliva_01.RAW	Raw
	Md_saliva_01.srf	Other
	Md_saliva_01.zip	Other
	Md_saliva_02.RAW	Raw