Proteomics

Dataset Information

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Metaproteomics of Premature Infant Gut Colonization


ABSTRACT: Microbial colonization of the human gastrointestinal tract plays an important role in establishing health and homeostasis. However, the time-dependent and related functional signatures of microbial and human proteins during early colonization of the gut have yet to be determined. Thus, we employed shotgun proteomics via nano-2D-LC-MS/MS to simultaneously monitor microbial and human proteins in fecal samples from a healthy preterm infant during early development. ). All MS/MS spectra were searched against a predicted protein database containing 25 microbial species along with the Human RefSeq2011 genome using the SEQUEST algorithm (Eng et al, 1994), and filtered with DTASelect version 1.9 (Tabb et al, 2002) at the peptide level with standard filters [SEQUEST Xcorrs of at least 1.8 (+1), 2.5 (+2) 3.5 (+3)] organizing identified peptides to their corresponding protein sequences. This study provides the first elucidation of coordinated human and microbial proteins in the infant gut during early development.

INSTRUMENT(S): LTQ Orbitrap Velos

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: Jacque Young  

LAB HEAD: Jacque Young

PROVIDER: PXD000114 | Pride | 2014-12-16

REPOSITORIES: Pride

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Publications

Metaproteomics reveals functional shifts in microbial and human proteins during a preterm infant gut colonization case.

Young Jacque C JC   Pan Chongle C   Adams Rachel M RM   Brooks Brandon B   Banfield Jillian F JF   Morowitz Michael J MJ   Hettich Robert L RL  

Proteomics 20150721 20


Microbial colonization of the human gastrointestinal tract plays an important role in establishing health and homeostasis. However, the time-dependent functional signatures of microbial and human proteins during early colonization of the gut have yet to be determined. To this end, we employed shotgun proteomics to simultaneously monitor microbial and human proteins in fecal samples from a preterm infant during the first month of life. Microbial community complexity increased over time, with comp  ...[more]

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