Proteomics

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Analysis of botrytis cinerea mycelium proteins

ABSTRACT: Comparison of Botrytis cinerea wild-type B.05.10 and T4 strains using label-free nUPLC-MSE and 2-DE approaches. B. cinerea strain mycelium were grown in synthetic minimal medium-modified Czapeck-Dox. Protein extracts were obtained from pulverized mycelium using TCA-phenol method. Acquired spectra were internally calibrated with peptides from trypsin 180 autolysis (M+H+=842.509,M+H+=2211.104) with an m/z precision of ±20 ppm. A combined search (PMF and MS/MS) was performed with GPS ExplorerTM software v3.5 (Applied Biosystems) over non-redundant NCBI databases using the MASCOT search engine. The database search utilized the following parameters: taxonomy restrictions to Fungi (06.17.2011), one missed cleavage sites, 100 ppm mass tolerance in MS and 0.5 Da for MS/MS data, cysteine carbamidomethylation as a fixed modification, and methionine oxidation as a variable modification. The confidence in the peptide mass fingerprinting matches (p<0.05) was based on the MOWSE score.

INSTRUMENT(S): Synapt MS, 4800 Proteomics Analyzer

ORGANISM(S): Botryotinia Fuckeliana (strain T4) (noble Rot Fungus) (botrytis Cinerea) Botryotinia Fuckeliana (strain B05.10) (noble Rot Fungus) (botrytis Cinerea)

SUBMITTER: Kerman Aloria  

PROVIDER: PXD000165 | Pride | 2013-05-02

REPOSITORIES: Pride

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Dataset's files

Source:
Action DRS
B_05_10_1.raw.zip Raw
B_05_10_1_APEX3D_PEPTIDE3D.zip Other
B_05_10_1_ION_ACCOUNTING.zip Other
B_05_10_2.raw.zip Raw
B_05_10_2_APEX3D_PEPTIDE3D.zip Other
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Publications

Application of label-free shotgun nUPLC-MS(E) and 2-DE approaches in the study of Botrytis cinerea mycelium.

Gonzalez-Fernandez Raquel R   Aloria Kerman K   Arizmendi Jesus M JM   Jorrin-Novo Jesus V JV  

Journal of proteome research 20130516 6

The phytopathogenic fungus Botrytis cinerea infects more than different 200 plant species and causes substantial losses in numerous crops. The B05.10 and T4 wild-type strain genomes have been recently sequenced, becoming a model system for necrotrophic pathogens, as well as opening up new alternatives in functional genomics, such as proteomics. We analyzed B. cinerea mycelium from these two wild-type strains, introducing label-free shotgun nUPLC-MS(E) methodology to complement the 2-DE-MS-based  ...[more]

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