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Cotton Fiber Glycoproteome - Glycoproteome of elongating cotton fiber cells

ABSTRACT: Gel based approach : Concanavalin A bound fiber glycoproteins were resolved through SDS PAGE followed by identification of trypsinised gel pieces through Nano-LC MALDI TOF/TOF. Solution based approach: Concanavalin A bound fiber glycoproteins were solution phase digested and fractionated through SCX followed by Nano-LC MALDI TOF/TOF based identification

INSTRUMENT(S): ultraflex

ORGANISM(S): Gossypium

SUBMITTER: Saravanan Kumar

PROVIDER: PXD000178 | Pride | 2014-03-18

REPOSITORIES: Pride

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Dataset's files

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			Action	DRS
	GP1DPAGEALLRUNS18032012_version6_0.mgf	Mgf
	Gel_based_approach.baf	Other
	Gel_based_approach_Unique_proteins.csv	Csv
	Gel_based_approach_peptides.xml	Xml
	GelbasedapproachCottonfiberglycoproteinsBTDX.xml	Xml

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Publications

Glycoproteome of elongating cotton fiber cells.

Kumar Saravanan S Kumar Krishan K Pandey Pankaj P Rajamani Vijayalakshmi V Padmalatha Kethireddy Venkata KV Dhandapani Gurusamy G Kanakachari Mogilicherla M Leelavathi Sadhu S Kumar Polumetla Ananda PA Reddy Vanga Siva VS

Molecular & cellular proteomics : MCP 20130909 12

Cotton ovule epidermal cell differentiation into long fibers primarily depends on wall-oriented processes such as loosening, elongation, remodeling, and maturation. Such processes are governed by cell wall bound structural proteins and interacting carbohydrate active enzymes. Glycosylation plays a major role in the structural, functional, and localization aspects of the cell wall and extracellular destined proteins. Elucidating the glycoproteome of fiber cells would reflect its wall composition ...[more]

PMID: 24019148

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Glycoproteome of Elongating cotton fiber cells

Project description:Glycoproteins isolated from elongating cotton fiber cells were analyzed using 2D-PAGE followed by MALDI TOF/TOF based protein identification. Protein isoforms were identified using Tandem mass spectrometry. Lectin based Glycopeptide enrichment strategy followed by PNGase catalyzed deglycosylation reaction was employed to assign potential N-linked glycosylation sites.

2015-11-25 | PXD002849 | Pride

Glycoproteome of elongating cotton fiber cells.

Project description:Cotton ovule epidermal cell differentiation into long fibers primarily depends on wall-oriented processes such as loosening, elongation, remodeling, and maturation. Such processes are governed by cell wall bound structural proteins and interacting carbohydrate active enzymes. Glycosylation plays a major role in the structural, functional, and localization aspects of the cell wall and extracellular destined proteins. Elucidating the glycoproteome of fiber cells would reflect its wall composition as well as compartmental requirement, which must be system specific. Following complementary proteomic approaches, we have identified 334 unique proteins comprising structural and regulatory families. Glycopeptide-based enrichment followed by deglycosylation with PNGase F and A revealed 92 unique peptides containing 106 formerly N-linked glycosylated sites from 67 unique proteins. Our results showed that structural proteins like arabinogalactans and carbohydrate active enzymes were relatively more abundant and showed stage- and isoform-specific expression patterns in the differentiating fiber cell. Furthermore, our data also revealed the presence of heterogeneous and novel forms of structural and regulatory glycoproteins. Comparative analysis with other plant glycoproteomes highlighted the unique composition of the fiber glycoproteome. The present study provides the first insight into the identity, abundance, diversity, and composition of the glycoproteome within single celled cotton fibers. The elucidated composition also indirectly provides clues about unicellular compartmental requirements underlying single cell differentiation.

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Delineating the glycoproteome of elongating cotton fiber cells.

Project description:The data presented here delineates the glycoproteome component in the elongating cotton fiber cells attained using complementary proteomic approaches followed by protein and N-linked glycosylation site identification (Kumar et al., 2013) [1]. Utilizing species specific protein sequence databases in proteomic approaches often leads to additional information that may not be obtained using cross-species databases. In this context we have reanalyzed our glycoproteome dataset with the Gossypium arboreum, Gossypium raimondii (version 2.0) and Gossypium hirsutum protein databases that has led to the identification of 21 N-linked glycosylation sites and 18 unique glycoproteins that were not reported in our previous study. The 1D PAGE and solution based glycoprotein identification data is publicly available at the ProteomeXchange Consortium via the PRIDE partner repository (Vizcaíno et al., 2013) [2] using the dataset identifier PXD000178 and the 2D PAGE based protein identification and glycopeptide approach based N-linked glycosylation site identification data is available at the ProteomeXchange Consortium via the PRIDE partner repository (Vizcaíno et al., 2013) [2] using the dataset identifier PXD002849.

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Cotton Fiber Development Time-course Analysis

Project description:To get glance to the events of the fast elongation and second cell wall synthesis stages of developing cotton fiber cells, we examined expression patterns of over 5000 genes by cDNA array from 3 to 18 DPA in a 3-day interval. RNAs from the 3-, 6-, 9-, 12-, 15-, and 18-DPA fibers were compared to the 9-DPA fiber RNA for time-course analysis. Four biological repeats were carried out including two dye-swap ones.

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Cotton Fiber Development Time-course Analysis

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Comparison of cotton fiber to ovule at 9 DPA

Project description:RNAs from the upland cotton 9-DPA fibers were compared to the 9-DPA fiber-detached ovule. RNAs from the upland cotton 9-DPA fibers were compared to the 9-DPA fiber-detached ovule.

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Activation of Arabidopsis seed hair development by cotton fiber-related genes

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Activation of Arabidopsis seed hair development by cotton fiber-related genes

Project description:This experiment was designed to investigate the molecular basis of cotton fiber cell initiation. 32,000 ESTs were sequenced from Gossypium hirsutum L. TM-1 immature ovules (GH_TMO) and developed cotton oligonucleotide microarrays containing ~23,000 unigenes. Transcriptome analyses were performed to compare gene expression changes in laser capture microdissected fiber cell initials (or epidermis) and inner ovules. The gene expression profiles of the fiber cell initials were compared with those of the inner ovules in each developmental stage prior to, right at, and shortly after the initiation of fiber cells. Many genes in various molecular function or biological processes were over- or under-represented between fibers and non-fiber tissues in each developmental stage, suggesting temporal regulation of gene expression during early stages of fiber development. For gene expression studies using a large set cotton oligo-microarray, 4 developmental stages were chosen. To study differential expression during fiber initiation, ovules at -2 DPA, 0 DPA, and 2 DPA were used. One of the fiber elongation stage tissues (7 DPA) was included. In each developmental stage, epidermis was separated from inner ovules and subjected to the hybridization. In addition, epidermis and ovule comparisons were performed individually with 0 DPA as a control point for comparison.

2011-11-02 | E-GEOD-17378 | biostudies-arrayexpress