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Glycoproteome of Elongating cotton fiber cells

ABSTRACT: Glycoproteins isolated from elongating cotton fiber cells were analyzed using 2D-PAGE followed by MALDI TOF/TOF based protein identification. Protein isoforms were identified using Tandem mass spectrometry. Lectin based Glycopeptide enrichment strategy followed by PNGase catalyzed deglycosylation reaction was employed to assign potential N-linked glycosylation sites.

INSTRUMENT(S): ultraflex

ORGANISM(S): Gossypium

TISSUE(S): Plant Cell, Cotton Fibre

SUBMITTER: Saravanan Kumar

LAB HEAD: Vanga Siva Reddy

PROVIDER: PXD002849 | Pride | 2015-11-25

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	0102PNGaseCompoundList.zip	Other
	03-11PNGaseCompoundList.zip	Other
	GP 01 Xylosidase.dat-pride.pride.mgf.gz	Mgf
	GP 01 Xylosidase.dat-pride.pride.mztab.gz	Mztab
	GP 02 Xylosidase.dat-pride.pride.mgf.gz	Mgf

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Publications

Glycoproteome of elongating cotton fiber cells.

Kumar Saravanan S Kumar Krishan K Pandey Pankaj P Rajamani Vijayalakshmi V Padmalatha Kethireddy Venkata KV Dhandapani Gurusamy G Kanakachari Mogilicherla M Leelavathi Sadhu S Kumar Polumetla Ananda PA Reddy Vanga Siva VS

Molecular & cellular proteomics : MCP 20130909 12

Cotton ovule epidermal cell differentiation into long fibers primarily depends on wall-oriented processes such as loosening, elongation, remodeling, and maturation. Such processes are governed by cell wall bound structural proteins and interacting carbohydrate active enzymes. Glycosylation plays a major role in the structural, functional, and localization aspects of the cell wall and extracellular destined proteins. Elucidating the glycoproteome of fiber cells would reflect its wall composition ...[more]

PMID: 24019148

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