Proteomics

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BHLH Transcription Factors that Facilitate K+ Uptake During Stomatal Opening are Repressed by Abscisic Acid through Phosphorylation


ABSTRACT: Stomata open in response to light and close following exposure to abscisic acid (ABA). They regulate gas exchange between plants and atmosphere, allowing plants to adapt to changing environmental conditions. ABA binding to receptors initiates a signaling cascade that involves protein phosphorylation. Here we show that ABA induced phosphorylation of three basic helix-loop-helix (bHLH) transcription factors, called AKSs (ABA-RESPONSIVE KINASE SUBSTRATES; AKS1, AKS2, AKS3), in Arabidopsis guard cells, and that they facilitated stomatal opening through the transcription of genes encoding inwardly-rectifying K+ channels. aks1aks2-1 double mutant plants showed decreases in light-induced stomatal opening, K+ accumulation in response to light, activity of inwardly-rectifying K+ channels, and transcription of genes encoding major inwardly-rectifying K+ channels without affecting ABA-mediated stomatal closure. Overexpression of POTASSIUM CHANNEL IN ARABIDOPSIS THALIANA 1 (KAT1), which encodes a major inwardly-rectifying K+ channel in guard cells, rescued the phenotype of aks1aks2-1 plants. AKS1 bound directly to the promoter of KAT1, an interaction that was attenuated after ABA-induced phosphorylation. The ABA agonist pyrabactin induced phosphorylation of AKSs. Our results demonstrate that the AKS family of bHLH transcription factors facilitates stomatal opening through transcription of genes encoding inwardly-rectifying K+ channels, and that ABA suppresses the activity of inwardly-rectifying K+ channel activity by triggering the phosphorylation of these transcription factors. Microarray data have been deposited in the Gene Expression Omnibus with accession number: GSE46574.

OTHER RELATED OMICS DATASETS IN: PRJNA201022

INSTRUMENT(S): LTQ

ORGANISM(S): Arabidopsis Thaliana (mouse-ear Cress)

TISSUE(S): Plant Cell

SUBMITTER: Y Takahashi  

LAB HEAD: Y Takahashi

PROVIDER: PXD000235 | Pride | 2013-06-28

REPOSITORIES: Pride

Dataset's files

Source:
Action DRS
43kd.RAW Raw
53kd.RAW Raw
PRIDE_Exp_Complete_Ac_29512.pride.mgf.gz Mgf
PRIDE_Exp_Complete_Ac_29512.pride.mztab.gz Mztab
PRIDE_Exp_Complete_Ac_29512.xml.gz Xml
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Publications

bHLH transcription factors that facilitate K⁺ uptake during stomatal opening are repressed by abscisic acid through phosphorylation.

Takahashi Yohei Y   Ebisu Yuta Y   Kinoshita Toshinori T   Doi Michio M   Okuma Eiji E   Murata Yoshiyuki Y   Shimazaki Ken-Ichiro K  

Science signaling 20130618 280


Stomata open in response to light and close after exposure to abscisic acid (ABA). They regulate gas exchange between plants and the atmosphere, enabling plants to adapt to changing environmental conditions. ABA binding to receptors initiates a signaling cascade that involves protein phosphorylation. We show that ABA induced the phosphorylation of three basic helix-loop-helix (bHLH) transcription factors, called AKSs (ABA-responsive kinase substrates; AKS1, AKS2, and AKS3), in Arabidopsis guard  ...[more]

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