Dataset Information

Proteomic analysis of fluids from the genital tract of ewes during the estrous cycle

ABSTRACT: Fluids samples of oviduct, uterus and cervical mucus were collected in sheep D0 (J0) and D10 (J10) in spontaneous (N) and synchronized (I) by flushing with PBS 1X. Proteins for each fluid were included in-gel (3-4 bands) followed by Coomassie Blue staining. After reduction and alkylation, proteins were in-gel digested with trypsin and peptide mixtures were analyzed by nanoLC-MS/MS using LTQ velos Orbitrap mass spectrometer. Raw data files were converted to MGF with Proteome Discoverer software (version 1.2; Thermo Fischer Scientific, San Jose, USA). Precursor mass range of 350-5000 Da and signal to noise ratio of 1.5 were the criteria used for generation of peak lists. In order to identify the proteins, the peptide and fragment masses obtained were matched automatically against the mammalia section of a locally maintained copy of nr NCBI (01/01/2013). MS/MS ion searches were performed using MASCOT Daemon and search engine (version 2.3; Matrix Science, London, UK). The parameters used for database searches include trypsin as a protease with allowed two missed cleavage, carbamidomethylcysteine (+57 Da), oxidation of methionine (+16) and N-terminal protein acetylation (+42) as variable modifications. The tolerance of the ions was set to 5 ppm for parent and 0.8 Da for fragment ion matches

INSTRUMENT(S): LTQ Orbitrap

ORGANISM(S): Ovis Aries

SUBMITTER: G HARICHAUX

PROVIDER: PXD000299 | Pride | 2015-11-05

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	12-0210_Druart_Uterus_J0N-Co_1a_ORBI856.raw.mzML	Mzml
	12-0210_Druart_Uterus_J0N-Co_2a_ORBI857.raw.mzML	Mzml
	12-0210_Druart_Uterus_J0N-Co_3a_ORBI858.raw.mzML	Mzml
	12-0210_Druart_Uterus_J10N-Co_1a_ORBI859.raw.mzML	Mzml
	12-0210_Druart_Uterus_J10N-Co_2a_ORBI860.raw.mzML	Mzml

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Publications

Proteomes of the Female Genital Tract During the Oestrous Cycle.

Soleilhavoup Clement C Riou Cindy C Tsikis Guillaume G Labas Valerie V Harichaux Gregoire G Kohnke Philippa P Reynaud Karine K de Graaf Simon P SP Gerard Nadine N Druart Xavier X

Molecular & cellular proteomics : MCP 20151030 1

The female genital tract includes several anatomical regions whose luminal fluids successively interact with gametes and embryos and are involved in the fertilisation and development processes. The luminal fluids from the inner cervix, the uterus and the oviduct were collected along the oestrous cycle at oestrus (Day 0 of the cycle) and during the luteal phase (Day 10) from adult cyclic ewes. The proteomes were assessed by GeLC-MS/MS and quantified by spectral counting. A set of 940 proteins wer ...[more]

PMID: 26518761

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