Dataset Information

Environmental stress affects the activity of metabolic and growth factor signaling networks and induces autophagy markers in breast cancer cell lines

ABSTRACT: The breast cancer cell line MCF7 was exposed to RT comditions for 15min, 2h or left in the incubator. Cells were harvested and lyzed. Samples were digested with trypsin and subjected to phosphoenrichment usin TiO2. Phosphopeptides were run in a LTQ-Orbitrap-XL. 4 biological replicates per condition were done. Peaks lists were generated with Mascot Distiller (version 2.3) in MGF format and Database searches were with Mascot Server (version 2.3) against the SwissProt database restricted to human sequences (release October 2012) and trypsin cleavage. Restrictions were 7ppm for parent ions and 0.8 Da for fragment masses. Allowed modifications were phosphorylation of Ser/Thr/Tyr, pyro-Glu (N-term) and methionine oxidation and one miss-cleavage allowed. Quantification was by label-free using peak heights of extracted ion chromatograms (XICs) constructed with narrow mass windows (7ppm) and time windows (1.5 minutes). Pescal, a computer program written in house, was used to automate the generation of XICs and to calculate peak heights.

INSTRUMENT(S): LTQ Orbitrap

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Epithelial Cell, Cell Culture

SUBMITTER: Pedro Casado-Izquierdo

LAB HEAD: Pedro Casado-Izquierdo

PROVIDER: PXD000472 | Pride | 2014-04-11

REPOSITORIES: Pride

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Environmental stress affects the activity of metabolic and growth factor signaling networks and induces autophagy markers in MCF7 breast cancer cells.

Casado Pedro P Bilanges Benoit B Rajeeve Vinothini V Vanhaesebroeck Bart B Cutillas Pedro R PR

Molecular & cellular proteomics : MCP 20140114 3

Phosphoproteomic techniques are contributing to our understanding of how signaling pathways interact and regulate biological processes. This technology is also being used to characterize how signaling networks are remodeled during disease progression and to identify biomarkers of signaling pathway activity and of responses to cancer therapy. A potential caveat in these studies is that phosphorylation is a very dynamic modification that can substantially change during the course of an experiment ...[more]

PMID: 24425749

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