Dataset Information

Endogenous S-nitrosoproteome in colorectal cancer patients

ABSTRACT: Total lysate of tumorous and adjacent normal tissues from each colorectal cancer patient are followed by S-alkylating biotin switch strategy. All biotinylated peptides are analyzed by label-free quantitation and LC-MS/MS analysis. All peak lists from 66 raw MS/MS spectra were processed to mgf format by Mascot Distiller v2.1.1.0. The datasets were batch-searched and combined searched by Mascor v2.2 against the Swissprot v 57.8 human database. PEO-iodoacetyl-biotin (C, +414 Da), Caebamidomethyl (C) and oxidation (M) were specified as variable modifications. Peptides were considered to be identified if the Mascot individual ion score was higher than the Mascot identity score (p less than 0.05). To evaluate the false discovery rate in protein identification, a decoy database search against a randomized decoy database created by Mascot using identical search parameters and validation criteria were also performed. The search results in Mascot were exported in eXtensive Markup Language (XML) data format.

INSTRUMENT(S): Q-Tof Premier

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: Yi-Ju Chen

PROVIDER: PXD000467 | Pride | 2014-08-20

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	100605_SNOCRC9WF3.xml	Xml
	F019321_100603SNOCRC9WF3.dat	Other
	YJC_100327SNOCRC_B11b372_N01.mgf	Mgf
	YJC_100327SNOCRC_B11b372_N01.mzXML	Mzxml
	YJC_100327SNOCRC_B11b372_N02.mgf	Mgf

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Publications

Decoding the s-nitrosoproteomic atlas in individualized human colorectal cancer tissues using a label-free quantitation strategy.

Chen Yi-Ju YJ Ching Wei-Chieh WC Chen Jinn-Shiun JS Lee Tzong-Yi TY Lu Cheng-Tsung CT Chou Hsiao-Chiao HC Lin Pei-Yi PY Khoo Kay-Hooi KH Chen Jenn-Han JH Chen Yu-Ju YJ

Journal of proteome research 20140804 11

The abnormal S-nitrosylation induced by the overexpression and activation of inducible nitric oxide synthase (iNOS) modulates many human diseases, such as inflammation and cancer. To delineate the pathophysiological S-nitrosoproteome in cancer patients, we report an individualized S-nitrosoproteomic strategy with a label-free method for the site-specific quantification of S-nitrosylation in paired tumor and adjacent normal tissues from 11 patients with colorectal cancer (CRC). This study provide ...[more]

PMID: 25040305

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