Dataset Information

Synechococcus elongatus PCC7942 proteome's cyclic rhythm by LC-MSMS

ABSTRACT: To uncover the variation in protein abundances during 48 hours under light and dark cycles (12:12-hours), we used quantitative proteomics, with TMT 6-plex isobaric labeling. We queried the S. elongatus proteome at ten different time points spanning a single 24 hour period, leading to 20 time points over the full 48 hour period.

INSTRUMENT(S): LTQ Orbitrap Velos

ORGANISM(S): Synechococcus Elongatus (strain Pcc 7942) (anacystis Nidulans R2)

TISSUE(S): Cell Suspension Culture

SUBMITTER: Ana C. Guerreiro

LAB HEAD: Albert J.R. Heck

PROVIDER: PXD000510 | Pride | 2014-04-02

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	F293816_2-10.dat-pride-filtered.pride.mgf.gz	Mgf
	F293816_2-10.dat-pride-filtered.pride.mztab.gz	Mztab
	F293816_2-10.dat-pride-filtered.xml.gz	Xml
	F293851_13-22.dat-pride-filtered.pride.mgf.gz	Mgf
	F293851_13-22.dat-pride-filtered.pride.mztab.gz	Mztab

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Publications

Daily rhythms in the cyanobacterium synechococcus elongatus probed by high-resolution mass spectrometry-based proteomics reveals a small defined set of cyclic proteins.

Guerreiro Ana C L AC Benevento Marco M Lehmann Robert R van Breukelen Bas B Post Harm H Giansanti Piero P Maarten Altelaar A F AF Axmann Ilka M IM Heck Albert J R AJ

Molecular & cellular proteomics : MCP 20140327 8

Circadian rhythms are self-sustained and adjustable cycles, typically entrained with light/dark and/or temperature cycles. These rhythms are present in animals, plants, fungi, and several bacteria. The central mechanism behind these "pacemakers" and the connection to the circadian regulated pathways are still poorly understood. The circadian rhythm of the cyanobacterium Synechococcus elongatus PCC 7942 (S. elongatus) is highly robust and controlled by only three proteins, KaiA, KaiB, and KaiC. T ...[more]

PMID: 24677030

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