Browse
Submit Data
Databases
API
Help

Dataset Information

377 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

RALF induced quantitative phosphoproteome changes in Arabidopsis thaliana

ABSTRACT: Arabidopsis seedlings were treated with 1 uM RALF peptide for 5 min to examine rapid protein phosphorylation changes. Phosphopeptides from the plasma membrane were identified and quantified using Orbitrap mass spectrometer.

OTHER RELATED OMICS DATASETS IN: E-MEXP-3994

INSTRUMENT(S): LTQ Orbitrap XL

ORGANISM(S): Arabidopsis Thaliana (mouse-ear Cress)

TISSUE(S): Plant Cell

SUBMITTER: Miyoshi Haruta

LAB HEAD: Miyoshi Haruta

PROVIDER: PXD000515 | Pride | 2016-12-06

REPOSITORIES: Pride

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	A_PM.RAW	Raw
	A_PM14NSearch.dat	Other
	B_PM.RAW	Raw
	B_PM14NSearch.dat	Other
	PRIDE_Exp_Complete_Ac_31655.pride.mgf.gz	Mgf

Items per page:

1 - 5 of 10

Publications

A peptide hormone and its receptor protein kinase regulate plant cell expansion.

Haruta Miyoshi M Sabat Grzegorz G Stecker Kelly K Minkoff Benjamin B BB Sussman Michael R MR

Science (New York, N.Y.) 20140101 6169

Plant cells are immobile; thus, plant growth and development depend on cell expansion rather than cell migration. The molecular mechanism by which the plasma membrane initiates changes in the cell expansion rate remains elusive. We found that a secreted peptide, RALF (rapid alkalinization factor), suppresses cell elongation of the primary root by activating the cell surface receptor FERONIA in Arabidopsis thaliana. A direct peptide-receptor interaction is supported by specific binding of RALF to ...[more]

PMID: 24458638

Similar Datasets

Touch-induced phosphoproteome in Arabidopsis thaliana (XL)

Project description:Touch-induced change in protein phosphorylation was studied using Arabidopsis seedlings.

2019-11-08 | PXD006180 | Pride

Touch-induced phosphoproteome in Arabidopsis thaliana (Q Exactive)

Project description:Touch-induced change in protein phosphorylation was studied using Arabidopsis seedlings.

2019-10-28 | PXD006181 | Pride

The interaction between glucose and cytokinin signal transduction pathway in Arabidopsis thaliana

Project description:Columbia (Col) seeds were sown on half-strength Murashige and Skoog (MS) medium, supplemented with 1% sucrose and 0.8% agar and grown vertically in culture room conditions. The 5-d-old homogenous seedlings were washed five times with sterile water and lastly with liquid half strength MS medium without sugar to remove residual exogenous sugar. In order to deplete internal sugars seedlings were grown in sugar free liquid half strength MS medium for 24 h in dark. Thereafter, the seedlings were treated with half-strength MS medium containing 0% G, 0% G + 1 uM BAP, 3% G, and 3% G + 1 uM BAP for 3 h in dark. RNA was extracted and microarray analysis was performed. Please note: G stands for glucose and BAP stands for 6-Benzylaminopurine (cytokinin)

2014-02-07 | E-MEXP-3827 | biostudies-arrayexpress

Transcript profiling of auxin/cytokinin crosstalk in the Arabidopsis primary root apex

Project description:AtIPT8/pga22 seedlings (gain-of-function mutant in Ws background; Sun et al. 2003, Plant Physiology 131, pp167-176) were grown on vertical plates for 7 days in LD. The seedlings were then incubated directly on the plate with medium containing 5 uM 17-beta-estradiol (for induction of the IPT8 gene) or 5 uM trans-zeatin for 12 and 24 h. 5 mm primary root tips were harvested from the seedlings and pooled for microarray analysis. Total RNA was isolated from the samples with the RNeasy Plant Mini Kit from Qiagen.

2010-04-20 | GSE20231 | GEO

iTRAQ-based proteomic analysis reveals genome-wide protein expression patterns in allohexaploid bread wheat in adaptation to potassium deficiency and an important role of jasmonic acid

Project description:Two-week old bread wheat seedlings hydroponically grown Hoagland solution were transferred to potassium (K+)-free conditions for 8 d, their root and leaf proteome profiles were assessed using iTRAQ proteome method, and NCBInr database combined with the recently published bread wheat genome information were used to analyze the identified protein species. Over 4,000 unique proteins were identified, 818 K+-responsive protein species showed significant abundance regulation. The most majority of the identified K+-responsive protein species had exact gene loci, and showed no global but tissue- and chromosome- dependent genome distributions. The identified protein species were associated with diverse functions and exhibited organ-specific differences. Most of identified protein species associated with hormone synthesis were the enzymes involved in the synthesis of jasmonic acid (JA). Allene oxide synthase (AOS), a key JA synthesis-related enzyme, was significantly induced in both root and leaf organs of K+-deficient wheat seedlings, and its overexpression in rice enhanced the tolerance to low K+ or K+ deficiency, increased contents of K+ and JA and transcription levels of some K+-responsive genes. However, rice AOS T-DNA inserted mutant (osaos) exhibited more sensitivity to K+ deficiency. K+ deficiency significantly increased abundance of a high affinity K+ transporter (TaAHAK1), TaAHAK1 transgenic rice seedlings markedly alleviated sensitivity to K+ deficiency, and K+ deficiency also upregulated expression of homologous OsHAK1 gene in TaAOS transgenic rice plants. These results suggested an essential role of JA in K+ deficiency and gave molecular insight into the responses of plant to K+ deficiency.

2017-10-09 | PXD003570 | Pride

Expression data for minimally invasive glioblastoma stem-like cell (GSC) plasma membrane markers

Project description:We compared whole genome expression profiles of GSCs with normal human cortex, human neural stem cells (hNSC) from fetal cortex, glioblastoma (GBM) primary, and recurrent tumors to find GSC-specific plasma membrane transcripts. All of the expression profiles were batch normalized by a robust multichip average (RMA) algorithm using Geospiza GeneSifter (PerkinElmer) online microarray database and analysis software. The data was then exported into Microsoft Office Excel 2010 and organized for GSC transcripts with raw intensity values 10 fold or higher over normal brain, hNSCs, GBM primary and recurrent tumor samples. The reverse sorting algorithm was done to obtain downregulated GSC trascripts.

2013-11-14 | E-GEOD-51822 | biostudies-arrayexpress

Arabidopsis thaliana gene expression changes upon chitosan and CAPE treatments

Project description:The phytopathological studies often deal with approaches involving the employment of high elicitor concentrations necessary to provoke responses that could be easily observed and quantified. We tested the range of gene expression changes for a low concentration chitosan elicitor treatment of seedlings, 0.001%, and we compared it with the higher one, 0.01%. We uncover both the quantitative and qualitative changes in gene expressions between the two treatments. Further on, we tested if the gene expression can be modified by the treatment with the defence-related CAPE peptide (10 uM). We discovered several interesting candidate genes specific for each of treatments, and combination of treatments, that would be worth of further more focused genetic studies.

2023-09-01 | GSE239633 | GEO

The Proteasome Stress Regulon is Controlled by a Pair of NAC Transcription Factors in Arabidopsis

Project description:We report the expression differences between 5DAG Arabidopsis Col-0, rpn10-1, and rpn12a-1 seedlings treated with 100 uM MG132 for 0, 3, or 24 hours.

2016-05-21 | GSE81668 | GEO

iSB09 elicits ABA-responsive gene expression in Solanum lycopersicum seedlings

Project description:To compare the genome-wide transcriptional effect of ABA and iSB09 in tomato plants, we performed RNA-seq analysis of mock-, 10 uM ABA- or 20 uM iSB09-treated plants. Differential gene expression analysis between mock- and ABA-treated or iSB09-treated seedlings was done with DESeq2 and genes with an absolute value of log2 fold change (log2FC) > 1 or (log2FC) < -1 and p-adjusted value (padj) < 0.05 were selected. iSB09 upregulated and downregulated genes represent a subset of the ABA-responsive genes, which reflects the activation of PYL1-like and PYL4-like ABA receptors in tomato seedlings.

2024-07-22 | GSE256238 | GEO

Combinatory microarray and SuperSAGE analyses identify pairing-dependently transcribed genes in Schistosoma mansoni males, such as follistatin [Microarray]

Project description:Transcriptional profiling of S. mansoni pairing-experienced males (EM) vs. pairing unexperienced males (UM) Two conditions (EM vs. UM) with three biological samples of EM and in parallel other three biological samples of UM. For each sample (three EM and three UM) were performed four technical replicas.

2013-09-09 | E-GEOD-44193 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data