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RALF induced quantitative phosphoproteome changes in Arabidopsis thaliana

ABSTRACT: Arabidopsis seedlings were treated with 1 uM RALF peptide for 5 min to examine rapid protein phosphorylation changes. Phosphopeptides from the plasma membrane were identified and quantified using Orbitrap mass spectrometer.

OTHER RELATED OMICS DATASETS IN: E-MEXP-3994

INSTRUMENT(S): LTQ Orbitrap XL

ORGANISM(S): Arabidopsis Thaliana (mouse-ear Cress)

TISSUE(S): Plant Cell

SUBMITTER: Miyoshi Haruta

LAB HEAD: Miyoshi Haruta

PROVIDER: PXD000515 | Pride | 2016-12-06

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	A_PM.RAW	Raw
	A_PM14NSearch.dat	Other
	B_PM.RAW	Raw
	B_PM14NSearch.dat	Other
	PRIDE_Exp_Complete_Ac_31655.pride.mgf.gz	Mgf

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Publications

A peptide hormone and its receptor protein kinase regulate plant cell expansion.

Haruta Miyoshi M Sabat Grzegorz G Stecker Kelly K Minkoff Benjamin B BB Sussman Michael R MR

Science (New York, N.Y.) 20140101 6169

Plant cells are immobile; thus, plant growth and development depend on cell expansion rather than cell migration. The molecular mechanism by which the plasma membrane initiates changes in the cell expansion rate remains elusive. We found that a secreted peptide, RALF (rapid alkalinization factor), suppresses cell elongation of the primary root by activating the cell surface receptor FERONIA in Arabidopsis thaliana. A direct peptide-receptor interaction is supported by specific binding of RALF to ...[more]

PMID: 24458638

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