Dataset Information

Proteomic Analysis of Rat Liver S9 Fraction by 2D-LC-MSMS

ABSTRACT: Liver plays a crucial role in numerous metabolic processes and therefore is the primary target of study in drug discovery and molecular toxicology. Devising an appropriate analytical strategy for an efficient analysis of the liver samples has been a major concern in proteomics. The aim of this study was to develop an LC-MS based analytical method for identification of liver proteins with adequate peptide-level confidence and protein sequence coverage. The present dataset will improve the existing knowledge on the proteins identified in rat liver S9 fraction.

INSTRUMENT(S): TripleTOF 5600

ORGANISM(S): Rattus Norvegicus (rat)

TISSUE(S): Hepatocyte, Liver

DISEASE(S): Disease Free

SUBMITTER: Lekha Sleno

LAB HEAD: Lekha Sleno

PROVIDER: PXD000717 | Pride | 2015-03-26

REPOSITORIES: Pride

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Dataset from proteomic analysis of rat, mouse, and human liver microsomes and S9 fractions.

Golizeh Makan M Schneider Christina C Ohlund Leanne B LB Sleno Lekha L

Data in brief 20150224

Rat, mouse and human liver microsomes and S9 fractions were analyzed using an optimized method combining ion exchange fractionation of digested peptides, and ultra-high performance liquid chromatography (UHPLC) coupled to high resolution tandem mass spectrometry (HR-MS/MS). The mass spectrometry proteomics data have been deposited to the ProteomeXchange Consortium (http://proteomecentral.proteomexchange.org) via the PRIDE partner repository (Vizcaíno et al., 2013 [1]) with the dataset identifier ...[more]

PMID: 26217725

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			Action	DRS
	RLS.group.gz	Other
	RLS.mgf	Mgf
	RLS.xml	Xml
	RLS__FDR.xlsx	Xlsx
	RSP_1_1.wiff	Wiff