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Identification of Drosophila centromere associated proteins

ABSTRACT: affinity purification-MS analysis of Drosophila centromeric chromatin

INSTRUMENT(S): LTQ Orbitrap

ORGANISM(S): Drosophila Melanogaster (fruit Fly)

TISSUE(S): Cell Culture, Early Embryonic Cell

SUBMITTER: Andreas Thomae

LAB HEAD: Axel Imhof

PROVIDER: PXD000758 | Pride | 2014-07-31

REPOSITORIES: Pride

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			Action	DRS
	Barthetal_Proteomics2014-2.zip	Other
	Barthetal_Proteomics2014_mqpar.xml	Xml
	SID1508_H3_3_1.raw	Raw
	SID1508_H3_3_2.raw	Raw
	SID1508_H3_3_3.raw	Raw

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Publications

Identification of novel Drosophila centromere-associated proteins.

Barth Teresa K TK Schade Georg O M GO Schmidt Andreas A Vetter Irene I Wirth Marc M Heun Patrick P Thomae Andreas W AW Imhof Axel A

Proteomics 20140714 19

Centromeres are chromosomal regions crucial for correct chromosome segregation during mitosis and meiosis. They are epigenetically defined by centromeric proteins such as the centromere-specific histone H3-variant centromere protein A (CENP-A). In humans, 16 additional proteins have been described to be constitutively associated with centromeres throughout the cell cycle, known as the constitutive centromere-associated network (CCAN). In contrast, only one additional constitutive centromeric pro ...[more]

PMID: 24841622

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Tripartite organization of centromeric chromatin in budding yeast

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2011-12-07 | E-GEOD-28298 | biostudies-arrayexpress

Density and methylation state of CpG dinucleotides define histone variant specific retention of nucleosomes in mouse spermatozoa

Project description:Nucleosomes are the principal packaging units of chromatin and critical for gene regulation and genome stability. In mammals, a subset of nucleosomes fail to be replaced by protamines during spermatogenesis and are retained in mature spermatozoa providing opportunities for paternal epigenetic transmission. In humans, the remaining 10% localize at regulatory elements of genes. To assess evolutionary conservation and to dissect the molecular logic underlying nucleosome retention, we determined the genome wide nucleosome occupancy in mouse spermatozoa that only contain 1% residual histones. In striking contrast to mammalian somatic cells and haploid round spermatids, we observe high enrichment of nucleosomes at CpG-rich sequences throughout the genome, at conserved regulatory sequences as well as at intra- and intergenic regions and repetitive DNA. This preferred occupancy occurs mutually exclusive with DNA methylation both in mouse and human sperm. At unmethylated CpG-rich sequences, residing nucleosomes are largely composed of the H3.3 histone variant, and trimethylated at lysine 4 (H3K4me3). Both canonical H3.1/H3.2 and H3.3 variant histones are present at promoters marked by Polycomb-mediated H3K27me3, which is strongly predictive for gene repression in pre-implantation embryos. Our data indicate important roles of DNA sequence composition, DNA methylation, variant H3.3 and canonical H3.1/H3.2 histones and associated modifications in nucleosome retention versus eviction during the histone-to-protamine remodeling process in elongating spermatids and potentially in epigenetic inheritance by nucleosomes between generations. Identification of histone, histone variant and histone modification states in round spermatids and sperm

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Centromere misdivision promotes centromeric sequence changes, de novo centromere formation, and centromere expansion

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H2A.Bbd localizes to DNA synthesis sites

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2014-05-18 | PXD000789 | Pride

Epigenome characterization at single base-pair resolution

Project description:We have combined standard micrococcal (MNase) digestion of nuclei with a modified protocol for construction paired-end DNA sequencing libraries to map both nucleosomes and subnucleosome-sized particles at single base-pair resolution throughout the budding yeast genome. We found that partially unwrapped nucleosomes and subnucleosome-sized particles can occupy the same position within a cell population, suggesting dynamic behavior. By varying the time of MNase digestion, we have been able to observe changes that reflect differential sensitivity of particles, including eviction of nucleosomes. Our protocol and mapping method provide a general strategy for characterizing full epigenomes. We used micrococcal nuclease mapping, chromatin immunoprecipitation and paired-end sequencing to determine the structure of yeast centromeres at single base-pair resolution.

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Sequential centromere shift via de novo formation in maize

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