Dataset Information

Positional proteomics identification of the natural substrates of cytosolic carboxypeptidases

ABSTRACT: Cytosolic carboxypeptidases (CCPs) constitute a new subfamily of M14 metallocarboxypeptidases associated to axonal regeneration and neuronal degeneration, amongst others. CCPs are deglutamylating enzymes, able to catalyze the shortening of polyglutamate side-chains and the gene-encoded C-termini of tubulin, telokin and myosin light chain kinase. The functions of these enzymes are not entirely understood, in part due to lack of information about C-terminal protein processing in the cell and its functional implications. By means of C-terminal COFRADIC, a positional proteomics approach, we searched for cellular substrates targets of CCP1, the most relevant member of this family. We here identified 7 new putative CCP1 protein substrates, including ribosomal proteins, translation factors and high mobility group proteins.

INSTRUMENT(S): LTQ Orbitrap Velos

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Hek-293ft Cell

SUBMITTER: Sebastian Tanco

LAB HEAD: Kris Gevaert

PROVIDER: PXD000834 | Pride | 2014-12-02

REPOSITORIES: Pride

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			Action	DRS
	V1804_1076_Cterm_Diff_hCCP1_28-32_1.raw	Raw
	V1805_1076_Cterm_Diff_hCCP1_28-32_2.raw	Raw
	V1806_1076_Cterm_Diff_hCCP1_28-32_3.raw	Raw
	V1807_1076_Cterm_Diff_hCCP1_28-32_4.raw	Raw
	V1808_1076_Cterm_Diff_hCCP1_28-32_5.raw	Raw

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Publications

C-terminomics screen for natural substrates of cytosolic carboxypeptidase 1 reveals processing of acidic protein C termini.

Tanco Sebastian S Tort Olivia O Demol Hans H Aviles Francesc Xavier FX Gevaert Kris K Van Damme Petra P Lorenzo Julia J

Molecular & cellular proteomics : MCP 20141107 1

Cytosolic carboxypeptidases (CCPs) constitute a new subfamily of M14 metallocarboxypeptidases associated to axonal regeneration and neuronal degeneration, among others. CCPs are deglutamylating enzymes, able to catalyze the shortening of polyglutamate side-chains and the gene-encoded C termini of tubulin, telokin, and myosin light chain kinase. The functions of these enzymes are not entirely understood, in part because of the lack of information about C-terminal protein processing in the cell an ...[more]

PMID: 25381060

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