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Identification of PM-localized proteins

ABSTRACT: In order to assess the quality of alleged PM identifications from Arabidopsis, PM-enriched fractions were compared to PM-depleted fractions using 18O isotopic labeling and mass spectrometry. The two samples submitted are biological replicates. Keywords: Protein Localization via MS Two biological replicates were analyzed in order to assess sample complexity. PM-derived vesicles were digested in 18O water and compared to PM-depleted fractions digested in 16O (natural abundance) water. Relative ion intensities were then used to calculate a heavy/lite ratio with proteins showing enrichment in the upper phase having a ratio greater than 1 or 0 on a log2 scale.

ORGANISM(S): Arabidopsis thaliana

SUBMITTER: Michael Sussman

PROVIDER: E-GEOD-2830 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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