Proteomics

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Proteomics analysis of the effect of Slf1 deletion on yeast response to oxidative stress via LC-MSMS

ABSTRACT: The mechanisms by which RNA-binding proteins control the translation of subsets of mRNAs are not yet clear. Slf1p is an atypical La motif containing protein (LARP). LARPs are members of a superfamily of RNA-binding proteins conserved across eukaryotes. Transcriptomics and RIP-Seq analysis suggested an effect of Slf1 on the amount of mRNA of genes involved in the response to oxidative stress. To quantify these effects at the protein level, we used label-free mass spectrometry to compare the proteomes of wild-type and slf1Δ mutant strains following oxidative stress conditions. This analysis identified several proteins which are normally induced in response to hydrogen peroxide, but where this increase is attenuated in the slf1Δ mutant.

INSTRUMENT(S): Progenesis LC-MS, Dionex instrument model, Mascot, LTQ Orbitrap Elite

ORGANISM(S): Saccharomyces Cerevisiae (baker's Yeast)

SUBMITTER: David Talavera  

LAB HEAD: Christopher Grant

PROVIDER: PXD000887 | Pride | 2015-03-12

REPOSITORIES: Pride

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The yeast La related protein Slf1p is a key activator of translation during the oxidative stress response.

Kershaw Christopher J CJ   Costello Joseph L JL   Castelli Lydia M LM   Talavera David D   Rowe William W   Sims Paul F G PF   Ashe Mark P MP   Hubbard Simon J SJ   Pavitt Graham D GD   Grant Chris M CM  

PLoS genetics 20150108 1

The mechanisms by which RNA-binding proteins control the translation of subsets of mRNAs are not yet clear. Slf1p and Sro9p are atypical-La motif containing proteins which are members of a superfamily of RNA-binding proteins conserved in eukaryotes. RIP-Seq analysis of these two yeast proteins identified overlapping and distinct sets of mRNA targets, including highly translated mRNAs such as those encoding ribosomal proteins. In paralell, transcriptome analysis of slf1Δ and sro9Δ mutant strains  ...[more]

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