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Secretome study for human hepatic model during entamoeba invasion

ABSTRACT: Investigations of human parasitic diseases depend on the availability of appropriate in vivo animal models and ex vivo experimental systems, and are particularly difficult for pathogens whose exclusive natural hosts are humans, as for Entamoeba histolytica, the protozoan parasite responsible for amoebiasis. We elaborated a human hepatic in vitro model consisting of cultured liver sinusoidal endothelial cells and hepatocytes in a 3D collagen-I environment. We have characterized the model's barrier function, its chemical and structural complexity and examined E. histolytica invasion. This study deals with secretome of hepatic model under various invasion conditions.

INSTRUMENT(S): LTQ Orbitrap Velos

ORGANISM(S): Bos Taurus (bovine) Homo Sapiens (human) Entamoeba Histolytica Hm-1:imss-a

SUBMITTER: gagan jhingan

LAB HEAD: Nancy Guillen

PROVIDER: PXD000893 | Pride | 2014-08-13

REPOSITORIES: Pride

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			Action	DRS
	2013-08-02_GAG_Top10_GJ200_collagen1A.raw	Raw
	2013-08-02_GAG_Top10_GJ200_collagen1A_Search_EntamoebaDB.msf	Msf
	2013-08-02_GAG_Top10_GJ200_collagen1A_Search_EntamoebaDB.pep.xml	Pepxml
	2013-08-02_GAG_Top10_GJ200_collagen1A_Search_bovineDB.msf	Msf
	2013-08-02_GAG_Top10_GJ200_collagen1A_Search_bovineDB.pep.xml	Pepxml

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Project description:Entamoeba histolytica is a protozoan parasite that causes colitis and liver abscesses. Several Entamoeba species and strains with differing levels of virulence have been identified. E. histolytica HM-1:IMSS is a virulent strain, E. histolytica Rahman is a nonvirulent strain, and Entamoeba dispar is a nonvirulent species. We used an E. histolytica DNA microarray consisting of 2,110 genes to assess the transcriptional differences between these species/strains with the goal of identifying genes whose expression correlated with a virulence phenotype. We found 415 genes expressed at lower levels in E. dispar and 32 genes with lower expression in E. histolytica Rahman than in E. histolytica HM-1:IMSS. Overall, 29 genes had decreased expression in both the nonvirulent species/strains than the virulent E. histolytica HM-1:IMSS. Interestingly, a number of genes with potential roles in stress response and virulence had decreased expression in either one or both nonvirulent Entamoeba species/strains. These included genes encoding Fe hydrogenase (9.m00419), peroxiredoxin (176.m00112), type A flavoprotein (6.m00467), lysozyme (6.m00454), sphingomyelinase C (29.m00231), and a hypothetical protein with homology to both a Plasmodium sporozoite threonine-asparagine-rich protein (STARP) and a streptococcal hemagglutinin (238.m00054). The function of these genes in Entamoeba and their specific roles in parasite virulence need to be determined. We also found that a number of the non-long-terminal-repeat retrotransposons (EhLINEs and EhSINEs), which have been shown to modulate gene expression and genomic evolution, had lower expression in the nonvirulent species/strains than in E. histolytica HM-1:IMSS. Our results, identifying expression profiles and patterns indicative of a virulence phenotype, may be useful in characterizing the transcriptional framework of virulence. A species experiment design type assays differences between distinct species. Keywords: species_design

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Secretome study for human hepatic model during entamoeba invasion

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